Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1
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Data
2020-03-01
Autores
Tamayo, Abel I. Balbin
Rizo, Laura S. López
de Armas, Madelin Blanco
Ferreira, Antonio Aparecido Pupim [UNESP]
Manzani, Danilo
Yamanaka, Hideko [UNESP]
Guas, Ana Margarita Esteva
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Resumo
This study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected.
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Palavras-chave
DNA biosensors, Genosensor, HIV-1, Hybridization, Impedimetric, Proviral DNA
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Microchemical Journal, v. 153.