Impacto da prenhez na deposição de colágeno em bexiga de ratas
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2021-06-08
Autores
Mota, Samara Rodrigues [UNESP]
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Universidade Estadual Paulista (Unesp)
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Introdução: Durante o período gestacional o corpo da mulher passa por diversas modificações, sendo o sistema urinário, um dos principais sistemas que sofre adaptações. Estudos pré-clínicos mostraram alterações funcionais na bexiga durante a gestação, porém há escassez de estudos sobre possíveis alterações morfológicas na bexiga induzidas pelo período gestacional. Objetivo: avaliar a deposição de colágeno, por meio de análise histológica, na bexiga de ratas prenhes. Metodologia: Aprovado pelo CEUA nº 007/2016. Com 90 dias de vida, de forma aleatória, ratas Wistar foram submetidas ao acasalamento e concluído esse processo, divididas em ratas virgens (GV) (n=10) e prenhes (GP) (n=10). No 21º dia de prenhez, amostras de bexiga foram obtidas para confecção de lâminas histológicas coradas com Tricrômico de Masson para análise das fibras colágenas. Paralelamente, foi realizada dissecção e confecção das lâminas histológicas das bexigas das ratas virgens. Foram analisadas em microscópio de luz (Olympus Corporation® / BX41TF) acoplado à câmera digital DP25-4 para análise morfológica. Para análise morfométrica da área de colágeno, 100 campos histológicos de cada grupo foram selecionados e analisados por meio do software CellSens Standard (Olympus Corporation®). Resultados: Na análise morfológica ambos os grupos apresentaram estrutura histológica habitual da bexiga. Na análise morfométrica, foi observado aumento na deposição de colágeno nas bexigas do grupo GP (337566±115563 µm2) em comparação ao grupo GV (242166±91189 µm2). Conclusão: A prenhez promoveu aumento na deposição de colágeno na bexiga de ratas, sugerindo que pode existir correlação entre alterações de colágeno na bexiga e possíveis disfunções vesicais durante o período gestacional.
Introduction: During the gestational period, the woman's body undergoes several changes, and the urinary system is one of the systems that undergoes adaptations. Preclinical studies showed changes in the bladder during pregnancy, but there are few studies about possible morphological changes in the bladder induced by pregnancy. Objective: to evaluate collagen deposition, through histological analysis, in the bladder of pregnant rats. Methodology: Approved by CEUA No. 007/2016. At 90 days of life, randomly, Wistar rats were subjected to mating and this process concluded, divided into virgin (GV) (n = 10) and pregnant (GP) (n = 10) rats. On the 21st day of pregnancy, bladder samples were obtained to make histological slides stained with Masson's Trichrome for collagen fiber analysis. Simultaneously, dissection and preparation of histological slides of the virgin rats' bladders were performed. They were analyzed in light microscope (Olympus Corporation® / BX41TF) for morphological analysis. For morphometric analysis of collagen area, 100 histological fields from each group were selected and analised using CellSens Standard software (Olympus Corporation®). Results: The morphological analysis of both groups showed usual histological structure of the bladder. In the morphometric analysis, an increase in collagen deposition was observed in the bladders of GP group (337566 ± 115563 µm2) compared to GV group (242166 ± 91189 µm2). Conclusion: The pregnancy promoted an increase in the deposition of collagen in the bladder of female rats, suggesting that there may be a correlation between changes in collagen in the bladder and possible bladder dysfunction during the gestational period.
Introduction: During the gestational period, the woman's body undergoes several changes, and the urinary system is one of the systems that undergoes adaptations. Preclinical studies showed changes in the bladder during pregnancy, but there are few studies about possible morphological changes in the bladder induced by pregnancy. Objective: to evaluate collagen deposition, through histological analysis, in the bladder of pregnant rats. Methodology: Approved by CEUA No. 007/2016. At 90 days of life, randomly, Wistar rats were subjected to mating and this process concluded, divided into virgin (GV) (n = 10) and pregnant (GP) (n = 10) rats. On the 21st day of pregnancy, bladder samples were obtained to make histological slides stained with Masson's Trichrome for collagen fiber analysis. Simultaneously, dissection and preparation of histological slides of the virgin rats' bladders were performed. They were analyzed in light microscope (Olympus Corporation® / BX41TF) for morphological analysis. For morphometric analysis of collagen area, 100 histological fields from each group were selected and analised using CellSens Standard software (Olympus Corporation®). Results: The morphological analysis of both groups showed usual histological structure of the bladder. In the morphometric analysis, an increase in collagen deposition was observed in the bladders of GP group (337566 ± 115563 µm2) compared to GV group (242166 ± 91189 µm2). Conclusion: The pregnancy promoted an increase in the deposition of collagen in the bladder of female rats, suggesting that there may be a correlation between changes in collagen in the bladder and possible bladder dysfunction during the gestational period.
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Palavras-chave
Prenhez, Bexiga urinária, Morfologia, Colágeno, Pregnancy, Urinary bladder, Morphology, Collagen