THE EVALUATION OF CLOTTING TIME IN BOVINE THROMBIN, REPTILASE ® , AND THROMBIN-LIKE FRACTION OF Crotalus durissus terrificus VENOM USING BOVINE, EQUINE, OVINE, BUBALINE AND HUMAN CRYOPRECIPITATES
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Data
1998
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Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Resumo
The objective of this study was to evaluate the effects of the thrombin-like fraction of Crotalus durissus terrificus venom, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> , and bovine thrombin of fibrinogen pools on bovine, equine, ovine, bubaline and human cryoprecipitates. The authors also made a comparative study between animal and human cryoprecipitates to see if any there was any possibility of future use in medicine. Fibrinogen levels in cryoprecipitate were studied using 48 blood samples obtained as follows:12 samples from humans, 9 from bovine, 10 from equine, 10 from ovine and 7 from bubaline. The results obtained showed average levels of 375.50 mg % for humans, 218.33 mg % for bovine, 240.80 mg % for equine, 267.70 mg % for ovine and 664.00 mg % for bubaline. Upon the formation of pools of human and animal fibrinogens, the following results were obtained: 435 mg % for humans, 444 mg % for bovine, 337 mg % for equine, 390 mg % for ovine and 530 mg % for bubaline. Statistical analysis (using the analysis of variance for entirely randomized experiment for the calculation of F statistics) demonstrated that the bubaline fibrinogen level was higher than that of human, and both were higher than those of ovine, equine, and bovine. Clotting times were determined using different dilutions of bovine thrombin, thrombin-like fraction of Crotalus durissus terrificus venom, and Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> . Comparing these clotting times, results for human and bovine were found to be very similar, whereas using equine, ovine, and bubaline the results above a dilution of 1:3 were markedly different. The results obtained permitted the following conclusions to be drawn show that: 1) bovine thrombin presented better interactivity with fibrinogen extracted both from human and bovine cryoprecipitates; 2) there was similar behavior when bovine thrombin was substituted for Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> and for the thrombin-like fraction of Crotalus durissus terrificus venom; 3) cryoprecipitate from bovine can, in special circumstances, substitute human cryoprecipitate in medical practice; 4) human and bovine cryoprecipitates can be used with both Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> and Crotalus durissus terrificus fractions using a dilution up to 1:5; 5) the use of bovine cryoprecipitate can be recommended using either bovine thrombin, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif> , or thrombin-like fraction of Crotalus durissus terrificus venom.
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Thrombin-like fraction, bovine thrombin, Reptilase<img SRC=http:/img/fbpe/jvat/v4n2/image119.gif>, cryoprecipitate
Como citar
Journal of Venomous Animals and Toxins. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 4, n. 2, p. 120-136, 1998.