Indução de poliploidia em protocormos de Cattleya nobilior
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Data
2022-07-08
Autores
Moreira, Larissa Frigui
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Universidade Estadual Paulista (Unesp)
Resumo
A exuberância da Cattleya nobilior vem garantindo a ela mais espaço e relevância no mercado ornamental. Porém características almejadas como tamanho das flores, bem como sua intensidade de cor, podem ser acentuadas quando presentes em plantas poliplóides. Sendo assim, esse trabalho teve como objetivo determinar o tempo e a concentração ideal de colchicina. Após a imersão, os protocormos foram transferidos para meio sólido contendo carvão, a fim de avaliar a sobrevivência e o desenvolvimento das plantas pelo período de 30, 60 e 90 dias. O resultado obtido foi três protocormos sobreviventes, caracterizando o período de 24 horas como o único não letal para essa espécie. As concentrações de colchicina nos protocormos sobreviventes foi de 0,075 g.L-1 e 0,5 g.L-1. Além disso, devido ao aumento da massa nuclear, observada em microscópio, é possível supor que a poliploidia foi atingida nos protocormos, tendo seu melhor resultado nos tratamentos de 24 e 48 horas.
The exuberance of Cattleya nobilior has guaranteed it more space and relevance in the ornamental market. However, desired characteristics such as flower size, as well as their color intensity, can be accentuated when present in polyploid plants. Therefore, this work aimed to determine the ideal time and concentration of colchicine. After immersion, the protocorms were transferred to a solid medium containing charcoal, in order to evaluate the survival and development of the plants for a period of 30, 60 and 90 days. The result obtained was three surviving protocorms, characterizing the 24 hour period as the only non-lethal period for this species. Colchicine concentrations in the surviving protocorms were 0.075 g.L-1 and 0.5 g.L-1. In addition, due to the increase in nuclear mass, observed under the microscope, it is possible to assume that polyploidy was reached in the protocorms, with the best result being in the 24 and 48 hour treatments.
The exuberance of Cattleya nobilior has guaranteed it more space and relevance in the ornamental market. However, desired characteristics such as flower size, as well as their color intensity, can be accentuated when present in polyploid plants. Therefore, this work aimed to determine the ideal time and concentration of colchicine. After immersion, the protocorms were transferred to a solid medium containing charcoal, in order to evaluate the survival and development of the plants for a period of 30, 60 and 90 days. The result obtained was three surviving protocorms, characterizing the 24 hour period as the only non-lethal period for this species. Colchicine concentrations in the surviving protocorms were 0.075 g.L-1 and 0.5 g.L-1. In addition, due to the increase in nuclear mass, observed under the microscope, it is possible to assume that polyploidy was reached in the protocorms, with the best result being in the 24 and 48 hour treatments.
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Palavras-chave
Cattleya nobilior, poliploidia, colchicina, polyploid, colchicine