In vitro Trypanocidal Activity of Phenolic Derivatives from Peperomia obtusifolia
Nenhuma Miniatura disponível
Data
2009-05-01
Autores
Mota, Jonas da Silva
Leite, Ana Cristina [UNESP]
Batista Junior, Joao Marcos [UNESP]
Lopez, Silvia Noeli [UNESP]
Ambrosio, Daniela Luz [UNESP]
Passerini, Gabriela Duo [UNESP]
Kato, Massuo Jorge
Bolzani, Vanderlan da Silva [UNESP]
Barretto Cicarelli, Regina Maria [UNESP]
Furlan, Maysa [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Georg Thieme Verlag Kg
Resumo
The trypanocidal activity of crude extracts and fractions from the leaves and stems of Peperomia obtusifolia (Piperaceae) was evaluated in vitro against the epimastigote forms of Trypanosoma cruzi. Bioactivity-guided fractionation of the most active extracts afforded seven known compounds, including three chromanes, two furofuran lignans and two flavone C-diglycosides. The most active compounds were the chromanes peperobtusin A and 3,4-dihydro-5-hydroxy-2,7-dimethyl-8-(2 ''-methyl-2 ''-butenyl)-2-(4'-methyl-1',3'-pentadienyl)-2H-1-benzopyran-6-carboxylic acid, with IC(50) values of 3.1 mu M (almost three times more active than the positive control benznidazole, IC(50) 10.4 mu M) and 27.0 mu M, respectively. Cytotoxicity assays using peritoneal murine macrophages indicated that the chromanes were not toxic at the level of the IC(50) for trypanocidal activity. This is the first report on the trypanocidal activity besides unspecific cytotoxicity of chromanes from Peperomia species. Additionally it represents the first time isolation of 3,4-dihydro5-hydroxy-2,7-dimethyl-8-(2 ''-methyl-2 ''-butenyl)-2-(4'-methyl-1',3'-pentadienyl)-2H-1-benzopyran-6-carboxylic acid from P. obtusifolia.
Descrição
Palavras-chave
chromanes, Peperomia obtusifolia, Piperaceae, Trypanosoma cruzi
Como citar
Planta Medica. Stuttgart: Georg Thieme Verlag Kg, v. 75, n. 6, p. 620-623, 2009.