Identification of Eimeria mitis and Eimeria praecox in broiler feces using polymerase chain reaction
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Data
2004-12-01
Autores
Meireles, Marcelo Vasconcelos [UNESP]
Roberto, LO
Riera, RF
Título da Revista
ISSN da Revista
Título de Volume
Editor
Fundação APINCO de Ciência e Tecnologia Avícolas
Resumo
There are few reports concerning the epidemiology of Eimeria praecox and Eimeria mitis in Brazil. In the present experiment, the polymerase chain reaction (PCR) was used to identify these species in 156 samples of broiler chicken feces from several Brazilian states and the Federal District. Oocysts present in feces samples were purified by sodium chloride flotation followed by addition of DNAzol reagent (Invitrogen®) for extraction of genomic DNA. DNA was precipitated and stored following DNAzol reagent manufacture's instructions. The primers and PCR conditions were as described by Schnitzler et al. (1999). In the 156 field samples analyzed by PCR, 70 and 45 were positive for E. praecox and E. mitis, respectively. In this study we have shown that DNA extraction using DNAzol followed by PCR can be a useful tool in epidemiological studies, since it provides fast and reliable detection of Eimeria sp. in field samples.
Descrição
Palavras-chave
diagnosis, Eimeria mitis, Eimeria praecox, epidemiology, polymerase chain reaction
Como citar
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 6, n. 4, p. 249-252, 2004.