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dc.contributor.authorRizzatti, ACS
dc.contributor.authorSandrim, V. C.
dc.contributor.authorJorge, J.
dc.contributor.authorTerenzi, H. F.
dc.contributor.authorPolizeli, MDTM
dc.date.accessioned2014-05-20T15:22:05Z
dc.date.available2014-05-20T15:22:05Z
dc.date.issued2004-02-01
dc.identifierhttp://dx.doi.org/10.1007/s10295-004-0120-2
dc.identifier.citationJournal of Industrial Microbiology & Biotechnology. Heidelberg: Springer-verlag Heidelberg, v. 31, n. 2, p. 88-93, 2004.
dc.identifier.issn1367-5435
dc.identifier.urihttp://hdl.handle.net/11449/33149
dc.description.abstractThis study reports on the effects of growth temperature on the secretion and some properties of the xylanase and beta-xylosidase activities produced by a thermotolerant Aspergillus phoenicis. Marked differences were observed when the organism was grown on xylan-supplemented medium at 25 degreesC or 42 degreesC. Production of xylanolytic enzymes reached maximum levels after 72 h of growth at 42 degreesC; and levels were three- to five-fold higher than at 25 degreesC. Secretion of xylanase and beta-xylosidase was also strongly stimulated at the higher temperature. The optimal temperature was 85 degreesC for extracellular and 90 degreesC for intracellular beta-xylosidase activity, independent of the growth temperature. The optimum temperature for extracellular xylanase increased from 50 degreesC to 55 degreesC when the fungus was cultivated at 42 degreesC. At the higher temperature, the xylanolytic enzymes produced by A. phoenicis showed increased thermo stability, with changes in the profiles of pH optima. The chromatographic profiles were distinct when samples obtained from cultures grown at different temperatures were eluted from DEAE-cellulose and Biogel P-60 columns.en
dc.format.extent88-93
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Industrial Microbiology & Biotechnology
dc.sourceWeb of Science
dc.subjectaspergillus phoenicispt
dc.subjectthermostabilitypt
dc.subjectgrowth temperaturept
dc.subjectxylanasept
dc.subjectbeta-d-xylosidasept
dc.titleInfluence of temperature on the properties of the xylanolytic enzymes of the thermotolerant fungus Aspergillus phoenicisen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniv São Paulo, Dept Biol, Fac Filosofia,Ciências & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, Brazil
dc.description.affiliationFac Estadual Paulista Julio Mesquita Filho, Inst Quim, BR-14800900 Araraquara, Brazil
dc.description.affiliationUnespFac Estadual Paulista Julio Mesquita Filho, Inst Quim, BR-14800900 Araraquara, Brazil
dc.identifier.doi10.1007/s10295-004-0120-2
dc.identifier.wosWOS:000220644800006
dc.rights.accessRightsAcesso restrito
unesp.author.orcid0000-0002-6168-7470[2]
unesp.author.orcid0000-0002-5026-6363[5]
dc.relation.ispartofjcr3.103
dc.relation.ispartofsjr1,107
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