Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography
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Data
2005-03-25
Autores
Carvalho, RSH
Ianzer, D. A.
Malavolta, L.
Rodrigues, M. M.
Cilli, Eduardo Maffud [UNESP]
Nakaie, C. R.
Título da Revista
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Editor
Elsevier B.V.
Resumo
This paper deals with an unusual application for a copolymer of styrene-1 % divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. The so-called aminomethyl resin (AMR), to date only employed for peptide synthesis, swelled appreciably in water and was used successfully to purify negatively charged peptides. By correlating swelling degree of beads with pH of the media, it was possible to estimate that the AMR amino group pK(a) is approximately 5.5. In addition, the synthesized acetyl-(NANP)(3)-AMR succeeded in the affinity interaction with large antibody molecules related to malaria transmission and raised previously against this dodecapeptide sequence. (C) 2004 Elsevier B.V. All rights reserved.
Descrição
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resin, peptide, polymer, ion-exchange chromatography, affinity chromatography
Como citar
Journal of Chromatography B-analytical Technologies In the Biomedical and Life Sciences. Amsterdam: Elsevier B.V., v. 817, n. 2, p. 231-238, 2005.