Testing transposable elements as genetic drive mechanisms using Drosophila P element constructs as a model system
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Data
1997-01-01
Autores
Carareto, CMA
Kim, W.
Wojciechowski, M. F.
O'Grady, P.
Prokchorova, A. V.
Silva, J. C.
Kidwell, M. G.
Título da Revista
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Editor
Kluwer Academic Publ
Resumo
The use of transposable elements (TEs) as genetic drive mechanisms was explored using Drosophila melanogaster as a model system. Alternative strategies, employing autonomous and nonautonomous P element constructs were compared for their efficiency in driving the ry(+) allele into populations homozygous for a ry(-) allele at the genomic rosy locus. Transformed flies were introduced at 1%, 5%, and 10% starting frequencies to establish a series of populations that were monitored over the course of 40 generations, using both phenotypic and molecular assays. The transposon-borne ry(+) marker allele spread rapidly in almost all populations when introduced at 5% and 10% seed frequencies, but 1% introductions frequently failed to become established. A similar initial rapid increase in frequency of the ry(+) transposon occurred in several control populations lacking a source of transposase. Constructs carrying ry(+) markers also increased to moderate frequencies in the absence of selection on the marker. The results of Southern and in situ hybridization studies indicated a strong inverse relationship between the degree of conservation of construct integrity and transposition frequency. These finding have relevance to possible future applications of transposons as genetic drive mechanisms.
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Drosophila melanogaster, P elements, population studies, transposable elements
Como citar
Genetica. Dordrecht: Kluwer Academic Publ, v. 101, n. 1, p. 13-33, 1997.