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dc.contributor.authorNwidu, Lucky Lebgosi
dc.contributor.authorEssien, Grace Emannuel
dc.contributor.authorNwafor, Paul Alozie
dc.contributor.authorVilegas, Wagner [UNESP]
dc.date.accessioned2014-05-20T15:31:34Z
dc.date.available2014-05-20T15:31:34Z
dc.date.issued2011-12-01
dc.identifierhttp://dx.doi.org/10.3109/13880209.2011.589854
dc.identifier.citationPharmaceutical Biology. London: Informa Healthcare, v. 49, n. 12, p. 1249-1256, 2011.
dc.identifier.issn1388-0209
dc.identifier.urihttp://hdl.handle.net/11449/40671
dc.description.abstractContext: Carpolobia lutea G. Don (Polygalaceae) leaf is reputable as an antidiarrheal agent among the Efik and Ibibio tribe of Akwa Ibom State, Nigeria. The crude extract is reported to show antidiarrheal and antiulcer effects in rodents.Objective: The isolation and characterization of drug molecules from the leaf fraction with antidiarrheal bioactivity and determination of mechanism of action are reported.Material and methods: Gradient extraction by maceration yielding n-hexane, chloroform, ethyl acetate, and ethanol fractions (770 mg/kg) were used to establish the fractions suitable for drug discovery. The antidiarrheal effect of the leaf fractions of Carpolobia lutea was evaluated using castor oil-induced diarrhea, castor oil-induced intestinal transit, and enteropooling.Results: Results indicate that all fractions produced a significant (p < 0.01-0.001) decrease in castor oil-induced diarrhea in rats. This effect was not antagonized by isosorbide dinitrate (150 mg/kg, p.o), diphenoxylate (5 x 10(-3) mg/kg p.o) and yohimbine (1 mg/kg, s.c.) except for the chloroform fraction. The ethyl acetate fraction produced 100% inhibition of intestinal transit, an effect greater than pure drug. Phytochemical analysis of the ethyl acetate fraction yielded polyphenolic compounds.Conclusion: The leaf fractions contain two types of antidiarrheal agents, one mediating its effect through alpha(1) - presynaptic adrenoceptor while the other does not. Polyphenols isolated may in part lend credence for observed antidiarrheal activity.en
dc.description.sponsorshipNiger Delta University
dc.description.sponsorshipKhana Local Government Council, Bori, Ogoni, Nigeria
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1249-1256
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofPharmaceutical Biology
dc.sourceWeb of Science
dc.subjectCarpolobia luteaen
dc.subjectPolygalaceaeen
dc.subjectantidiarrhealen
dc.subjectmechanismen
dc.subjectisolationen
dc.subjectpolyphenolsen
dc.titleAntidiarrheal mechanism of Carpolobia lutea leaf fractions in ratsen
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dcterms.rightsHolderInforma Healthcare
dc.contributor.institutionNiger Delta Univ
dc.contributor.institutionUniv Uyo
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationNiger Delta Univ, Fac Pharm, Dept Pharmacol & Toxicol, Wilberforce Isl, Bayelsa State, Nigeria
dc.description.affiliationUniv Uyo, Fac Pharm, Dept Pharmacol & Toxicol, Uyo, Akwa Ibom State, Nigeria
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Organ Chem, Inst Chem, BR-14801970 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Organ Chem, Inst Chem, BR-14801970 São Paulo, Brazil
dc.identifier.doi10.3109/13880209.2011.589854
dc.identifier.wosWOS:000296981800005
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
dc.identifier.orcid0000-0003-3032-2556
unesp.author.orcid0000-0003-3032-2556[4]
dc.relation.ispartofjcr1.918
dc.relation.ispartofsjr0,563
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