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dc.contributor.authorGomes, Anderson J.
dc.contributor.authorLunardi, Claure N.
dc.contributor.authorLunardi, Laurelucia O.
dc.contributor.authorPitol, Dimitrius L.
dc.contributor.authorMachado, Antonio Eduardo H.
dc.date.accessioned2014-05-20T15:33:21Z
dc.date.available2014-05-20T15:33:21Z
dc.date.issued2008-01-01
dc.identifierhttp://dx.doi.org/10.1016/j.micron.2007.06.015
dc.identifier.citationMicron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 39, n. 1, p. 40-44, 2008.
dc.identifier.issn0968-4328
dc.identifier.urihttp://hdl.handle.net/11449/42007
dc.description.abstractDrug delivery systems involving the use of polymers are widely studied and discovery of biocompatible polymers has become the focus of research in this area. Psoralen loaded poly(DL-lactide-co-glycolide) (PLGA) microspheres to be used in PUVA therapy (psoralen and UVA irradiation (ultraviolet A, 320-400 nm) of psoriasis were identified in paraffin sections by histological analysis. The psoralen loaded PLGA microspheres were prepared using the solvent evaporation technique. They were spherical and possessed an external smooth surface as observed by scanning electron microscopy (SEM) analysis. This study describes a modification in the routine preparation of microsphere samples for examination by light microscopy. The changes involved fixative agents and/or stains allowing the identification of microspheres containing a non-fluorescent material. The preservation and identification of microspheres in tissues for histological processing in paraffin was greatly improved by these modifications as proven by our results. (c) 2007 Elsevicr Ltd. All rights reserved.en
dc.format.extent40-44
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofMicron
dc.sourceWeb of Science
dc.subjectdrug deliveryen
dc.subjectlight microscopyen
dc.subjectmicrospheresen
dc.subjectPLGAen
dc.subjectpsoralenen
dc.subjectrat skinen
dc.subjectirradiationen
dc.subjectstainen
dc.titleIdentification of psoralen loaded PLGA microspheres in rat skin by light microscopyen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionFac Ciencias Farmaceut Ribeirao Preto
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniversidade Federal de Uberlândia (UFU), Inst Quim, Lab Fotoquim, BR-38400089 Uberlandia, MG, Brazil
dc.description.affiliationFac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Julio Mesquita Filho, Inst Biociencia, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Julio Mesquita Filho, Inst Biociencia, BR-13506900 Rio Claro, SP, Brazil
dc.identifier.doi10.1016/j.micron.2007.06.015
dc.identifier.wosWOS:000251486300006
dc.rights.accessRightsAcesso restrito
dc.relation.ispartofjcr1.728
dc.relation.ispartofsjr0,624
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