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dc.contributor.authorSilva, Adriana Navarro da [UNESP]
dc.contributor.authorGarcia-Cruz, Crispin Humberto [UNESP]
dc.date.accessioned2014-05-20T15:34:24Z
dc.date.available2014-05-20T15:34:24Z
dc.date.issued2010-01-01
dc.identifierhttp://dx.doi.org/10.4025/actascitechnol.v32i2.1792
dc.identifier.citationActa Scientiarum-technology. Maringa: Universidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacao, v. 32, n. 2, p. 105-112, 2010.
dc.identifier.issn1806-2563
dc.identifier.urihttp://hdl.handle.net/11449/42540
dc.description.abstractThe response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii. Alginate is a polysaccharide extracted from cell walls of brown algae and used in the food, pharmaceuticals and biotech industries. Production is concentrated on the cultivation of brown seaweed, but several bacteria of the genus Pseudomonas and Azotobacter produce alginate. The chemical structure of alginates produced by algae is similar to those synthesized by A. vinelandii. The bacteria also produce intracellular polymers such as polyhydroxybutyrate (PHB), known as bioplastic. This work studied the simultaneous alginate and PHB production by A. vinelandii using sucrose and different parameters of fermentation in an orbital shaker. The optimal values for the production of these compounds were determined by the MSR. The first experiment was a 2(6-2) factorial design. The second was based on significant variables of the first, resulting in a full 3(3-0) factorial design. From the first to the second, an increase was observed in the PHB productivity from 12 to 45 mg g(-1) cell h(-1) and alginate from 100 to 1,600 mg g(-1) of cell h(-1). The productivity of both compounds was in the maximum incubation temperature of 62 degrees C, in the shortest time of incubation (18h) and the sucrose concentration, 11 g L(-1). In both experiments the PHB extracted presented purity of 94%.en
dc.format.extent105-112
dc.language.isopor
dc.publisherUniversidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacao
dc.relation.ispartofActa Scientiarum: Technology
dc.sourceWeb of Science
dc.subjectalginateen
dc.subjectpolyhydroxybutyrate (PHB)en
dc.subjectproductionen
dc.subjectAzotobacter vinelandiien
dc.titleA metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandiipt
dc.title.alternativeThe response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandiien
dc.typeResumo
dcterms.licensehttp://periodicos.uem.br/ojs/index.php/ActaSciTechnol/about/editorialPolicies#openAccessPolicy
dcterms.rightsHolderUniversidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacao
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv Estadual Paulista, BR-15054000 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, BR-15054000 São Paulo, Brazil
dc.identifier.doi10.4025/actascitechnol.v32i2.1792
dc.identifier.wosWOS:000282011100001
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
dc.identifier.fileWOS000282011100001.pdf
dc.relation.ispartofjcr0.231
dc.relation.ispartofsjr0,168
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