Genetic diversity of two Brazilian populations of the Pampas deer (Ozotoceros bezoarticus, Linnaeus 1758)
Carregando...
Data
2007-12-01
Autores
Rodrigues, FP. [UNESP]
Garcia, JF. [UNESP]
Ramos, PRR. [UNESP]
Bortolozzi, J. [UNESP]
Duarte, JMB. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Instituto Internacional de Ecologia
Resumo
O Veado-campeiro (Ozotoceros bezoarticus) é uma das espécies de cervídeos neotropical mais ameaçadas devido à destruição de seu hábitat e conseqüente redução e isolamento de suas populações. Marcadores do tipo RAPD (Random amplified polymorphic DNA) foram utilizados na análise da divergência populacional e estimativa da variação genética dentro e entre duas populações correspondentes a diferentes subespécies. Os marcadores RAPD mostraram uma variação genética substancial, sendo que as 105 bandas polimórficas obtidas pelo uso de 15 primers produziram fenótipos únicos para todos os indivíduos analisados. Para avaliar o nível de diferenciação entre as populações, foi realizada uma análise da variância molecular (AMOVA) e uma análise de agrupamento utilizando o método de neighbor-joining. Nenhuma diferenciação foi observada, sendo aproximadamente 96,0% da variação encontrada atribuída à variação dentro das populações estudadas. Este resultado difere do obtido através da análise da região controle do mtDNA, e é discutido levando-se em consideração as diferenças genéticas entre os diferentes marcadores utilizados e o padrão de dispersão geralmente observado nas espécies de mamíferos (realizada principalmente pelos machos). Os dados aqui apresentados poderão ser úteis para futuros estudos taxonômicos e genéticos desta espécie, para o monitoramento da variação genética observada em suas populações e para o desenvolvimento de estratégias de manejo para sua conservação.
The Pampas deer (Ozotoceros bezoarticus) is one of the most endangered Neotropical cervid with populations that have been drastically reduced to small and isolated ones, mainly because of its habitat destruction. Random amplified polymorphic DNA (RAPD) markers were used to analyze population divergence and genetic variation within and between two populations corresponding to distinct subspecies. The RAPD markers displayed substantial genetic variation with all animals possessing unique RAPD phenotypes over 105 polymorphic bands produced by 15 primers. An analysis of molecular variance (AMOVA) and a neighbor-joining cluster analysis were performed to assess levels of differentiation between populations. No differentiation was recorded and about 96.0% (P < 0.00001) of the total variance was attributable to variation within populations. This result is quite distinct from data obtained by the analysis of the mtDNA control region, and is discussed on the basis of genetic differences between the different markers and the male-biased dispersal patterns generally observed in the mammal species. The data presented herein are potentially useful for future taxonomic and genetic studies in this species, for the monitoring of the genetic variation observed within these populations, and for the development of management guidelines for its conservation.
The Pampas deer (Ozotoceros bezoarticus) is one of the most endangered Neotropical cervid with populations that have been drastically reduced to small and isolated ones, mainly because of its habitat destruction. Random amplified polymorphic DNA (RAPD) markers were used to analyze population divergence and genetic variation within and between two populations corresponding to distinct subspecies. The RAPD markers displayed substantial genetic variation with all animals possessing unique RAPD phenotypes over 105 polymorphic bands produced by 15 primers. An analysis of molecular variance (AMOVA) and a neighbor-joining cluster analysis were performed to assess levels of differentiation between populations. No differentiation was recorded and about 96.0% (P < 0.00001) of the total variance was attributable to variation within populations. This result is quite distinct from data obtained by the analysis of the mtDNA control region, and is discussed on the basis of genetic differences between the different markers and the male-biased dispersal patterns generally observed in the mammal species. The data presented herein are potentially useful for future taxonomic and genetic studies in this species, for the monitoring of the genetic variation observed within these populations, and for the development of management guidelines for its conservation.
Descrição
Palavras-chave
Cervidae, Ozotoceros bezoarticus, RAPD, Genetic diversity, population structure, Cervidae, Ozotoceros bezoarticus, RAPD, Diversidade genética, Estrutura populacional
Como citar
Brazilian Journal of Biology. Instituto Internacional de Ecologia, v. 67, n. 4, p. 805-811, 2007.