Histochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical training
MetadataShow full item record
Alterations in liver functions are common among diabetic patients, and many symptoms in the liver have been reported, including changes in glycogen stores and in the amount of collagen fibers. The practice of physical training and its morphological effects in this organ, however, are scarcely studied. In order to observe the morphological effects of alloxan-induced diabetes and the alterations arising from the practice of long-term chronic physical training in the liver, samples were collected and processed, and then analyzed by means of the histochemical techniques Periodic Acid-Schiff and Picrosirius-Hematoxylin, and ultrastructural cytochemical test of Afzelius. Through evaluation of the tissue, it was observed a drastic reduction in hepatic glycogen stores of sedentary diabetics, recovered in trained diabetic rats. Furthermore, it was detected a decrease in the content of perisinusoidal collagen fibers in the diabetic liver, also recovered due to the development of a training protocol. On ultrastructural level, cytochemical analysis confirmed the loss of glycogen and the recovery obtained by training. In conclusion, the practice of a long-term chronic physical training protocol may be considered an important assistant in the treatment of diabetes, mitigating the occurrence of possible damages to liver tissue. © 2011 Elsevier Ltd.
How to cite this document
Showing items related by title, author, creator and subject.
Batista, M. R.; Curi, R.; Lima, F. B.; Lopes, G.; Bazotte, R. B. (Journal of Biomedical Science, 2001) [Artigo]We investigated the effect of a meal feeding schedule (MFS) on food intake, hepatic glycogen synthesis, hepatic capacity to produce glucose and glycemia in rats. The MFS comprised free access to food for a 2-hour period ...
Furuya, Daniela Tomie; Binsack, Ralf; Onishi, Mary Emy; Seraphim, Patricia Monteiro; Machado, Ubiratan Fabres (Life Sciences, 2005) [Artigo]Moderate amounts of alcohol intake have been reported to have a protective effect on the cardiovascular system and this may involve enhanced insulin sensitivity. We established an animal model of increased insulin sensitivity ...
Monitoring chronic physical stress using biomarkers, performance protocols and mathematical functions to identify physiological adaptations in rats de Araujo, Gustavo Gomes; Papoti, Marcelo; de Barros Manchado-Gobatto, Fúlvia; de Mello, Maria Alice Rostom ; Gobatto, Claudio Alexandre (Laboratory Animals, 2013) [Artigo]This study was undertaken to characterize the effects of monotonous training at lactate minimum (LM) intensity on aerobic and anaerobic performances; glycogen concentrationsin the soleus muscle, the gastrocnemius muscle ...