Supplementation with the histone deacetylase inhibitor trichostatin A during in vitro culture of bovine embryos
Data de publicação2013-02-01
Direito de acesso
MetadadosExibir registro completo
Summary Trichostatin A (TSA) is a histone deacetylase inhibitor that induces histone hyperacetylation and increases gene expression levels. The aim of the present study was to establish a suitable condition for the use of TSA in in vitro cultures of bovine embryos, and to determine whether TSA would increase blastocyst rates by improvement of chromatin remodelling during embryonic genome activation and by increasing the expression of crucial genes during early development. To test this hypothesis, 8-cell embryos were exposed to four concentrations of TSA for different periods of time to establish adequate protocols. In a second experiment, three experimental groups were selected for the evaluation of embryo quality based on the following parameters: apoptosis, total cell number and blastocyst hatching. TSA promoted embryonic arrest and degeneration at concentrations of 15, 25 and 50 nM. All treated groups presented lower blastocyst rates. Exposure of embryos to 5 nM for 144 h and to 15 nM for 48 h decreased blastocyst hatching. However, the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay (TUNEL) assay revealed similar apoptosis rates and total cell numbers in all groups studied. Although, in the present study, TSA treatment did not improve the parameters studied, the results provided background information on TSA supplementation during in vitro culture of bovine embryos and showed that embryo quality was apparently not affected, despite a decrease in blastocyst rate after exposure to TSA. © Cambridge University Press 2011.
Como citar este documento
Este item aparece nas seguintes coleções
Exibindo os itens relacionados pelo título, autor e palavra-chave.
Oliveira, Clara Slade ; Saraiva, Naiara Zoccal ; Cruz, Maria Helena Coelho ; Mazeti, Bruna ; Oliveira, Leticia Zoccolaro ; Lopes, Flavia Lombardi ; Garcia, Joaquim Mansano (Reproduction, 2013) [Artigo]During initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing ...
Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos Cardoso, Tereza Cristina; Silva-Frade, Camila ; Táparo, Cilene Vidovix ; Okamura, Lucas Hidenori ; Flores, Eduardo Furtado (Molecular and Cellular Probes, 2013) [Artigo]The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed ...
Gaspar, Roberta C. ; Arnold, Daniel R.; Correa, Carolina A. P. ; Rocha, Carlos V. da ; Penteado, Joao C. T. ; del Collado, Maite ; Vantini, Roberta ; Garcia, Joaquim M. ; Lopes, Flavia L. (Theriogenology, 2015) [Artigo]In vitro production of bovine embryos is a biotechnology of great economic impact. Epigenetic processes, such as histone remodeling, control gene expression and are essential for proper embryo development. Given the ...