Evaluation epidermal p53 immunostaining by digital image analysis
MetadataShow full item record
Digital techniques have been developed and validated to assess semiquantitatively immunohistochemical nuclear staining. Currently visual classification is the standard for qualitative nuclear evaluation. Analysis of pixels that represents the immunohistochemical labeling can be more sensitive, reproducible and objective than visual grading. This study compared two semiquantitative techniques of digital image analysis with three techniques of visual analysis imaging to estimate the p53 nuclear immunostaining. Methods: Sixty-three sun-exposed forearm-skin biopsies were photographed and submitted to three visual analyses of images: the qualitative visual evaluation method (0 to 4 +), the percentage of labeled nuclei and HSCORE. Digital image analysis was performed using ImageJ 1.45p; the density of nuclei was scored per ephitelial area (DensNU) and the pixel density was established in marked suprabasal epithelium (DensPSB). Results: Statistical significance was found in: the agreement and correlation among the visual estimates of evaluators, correlation among the median visual score of the evaluators, the HSCORE and the percentage of marked nuclei with the DensNU and DensPSB estimates. DensNU was strongly correlated to the percentage of p53-marked nuclei in the epidermis, and DensPSB with the HSCORE. Conclusion: The parameters presented herein can be applied in routine analysis of immunohistochemical nuclear staining of epidermis. © 2012 John Wiley & Sons A/S.
How to cite this document
Showing items related by title, author, creator and subject.
De Oliveira, Maria Cristina Ferreira; Shimabukuro, Milton Hirokazu (Proceedings of SPIE - The International Society for Optical Engineering, 2004) [Trabalho apresentado em evento]Interactive visual representations complement traditional statistical and machine learning techniques for data analysis, allowing users to play a more active role in a knowledge discovery process and making the whole process ...
Afonso, L. ; Papa, J. ; Papa, L. ; Marana, Aparecido Nilceu ; Rocha, Anderson (Proceedings - International Conference on Image Processing, ICIP, 2012) [Trabalho apresentado em evento]Image categorization by means of bag of visual words has received increasing attention by the image processing and vision communities in the last years. In these approaches, each image is represented by invariant points ...
Shimabukuro, M. H.; Flores, E. F.; de Oliveira, MCF; Levkowitz, H. (Second International Conference on Coordinated & Multiple Views In Exploratory Visualization, Proceedings, 2004) [Trabalho apresentado em evento]In this paper we focus on providing coordinated visual strategies to assist users in performing tasks driven by the presence of temporal and spatial attributes. We introduce temporal visualization techniques targeted at ...