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dc.contributor.authorFerreira, A. A. P.
dc.contributor.authorColli, W.
dc.contributor.authorAlves, M. J. M.
dc.contributor.authorOliveira, D. R.
dc.contributor.authorCosta, P. I.
dc.contributor.authorGuell, A. G.
dc.contributor.authorSanz, F.
dc.contributor.authorBenedetti, Assis Vicente [UNESP]
dc.contributor.authorYamanaka, Hideko [UNESP]
dc.identifier.citationElectrochimica Acta. Oxford: Pergamon-Elsevier B.V., v. 51, n. 24, p. 5046-5052, 2006.
dc.description.abstractThis present work reports on development of an amperometric immunosensor for the diagnosis of Chagas' disease using a specific glycoprotein of the trypomastigote surface, which belongs to the Tc85-11 protein family of Trypanosoma cruzi (T cruzi). An atomically flat gold surface on a silicon substrate and gold screen-printed electrodes were functionalized with cystatrine and later activated with glutaraldehyde (GA), which was used to form covalent bonds with the purified recombinant antigen (Tc85-11). The antigen reacts with the antibody from the serum, and the affinity reaction was monitored directly using atomic force microscopy or amperometry through a secondary antibody tagged to peroxidase (HRP). Surface imaging allowed to us to differentiate the modification steps and antigen-antibody interaction allowed to distinguish the affinity reactions. In the amperometric immunosensor, peroxidase catalyses the L-2 formation in the presence of hydrogen peroxide and potassium iodide, and the reduction current intensity was measured at a given potential with screen-printed electrodes. The immunosensor was applied to sera of chagasic patients and patients having different systemic diseases. (c) 2006 Elsevier Ltd. All rights reserved.en
dc.publisherElsevier B.V.
dc.relation.ispartofElectrochimica Acta
dc.sourceWeb of Science
dc.subjectChagas' diseasept
dc.subjectTc85-11 proteinpt
dc.subjectamperometric immunosensorpt
dc.titleInvestigation of the interaction between Tc85-11 protein and antibody anti-T cruzi by AFM and amperometric measurementsen
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Barcelona
dc.description.affiliationUNESP, Inst Chem, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Chem, BR-05508900 São Paulo, Brazil
dc.description.affiliationUNESP, Fac Pharmaceut Sci, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUniv Barcelona, CBEN, E-08028 Barcelona, Spain
dc.description.affiliationUnespUNESP, Inst Chem, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Pharmaceut Sci, BR-14801970 Araraquara, SP, Brazil
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
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