Influência da vitamina E na cinética do processo cicatricial induzido em tilápia do Nilo, Oreochromis niloticus
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Data
2008-08-22
Autores
Iwashita, Marina Keiko Pieroni [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
A vitamina E ou tocoferol é um importante nutriente que atua como imunoestimulante em peixes. No presente estudo foi verificado o efeito deste nutricêutico no processo de cicatrização induzido em tilápia do Nilo, suplementadas ou não com 450 mg de vitamina E. Exemplares desta espécie foram separados em dois grupos contendo 90 peixes, denominados controle e suplementados, distribuídos em 18 caixas de água com capacidade de 250L com 10 exemplares em cada. Os peixes foram alimentados durante 60 dias com as rações experimentais. Após este período, todos os peixes foram anestesiados e submetidos às cirurgias, onde feridas cutâneas de tamanho padronizado e representativas do processo cicatricial foram realizadas cirurgicamente com remoção da epiderme e derme. A análise histomorfométrica foi verificada após três, sete, 14, 21 e 28 dias. Foram utilizados como parâmetros para análise macroscópica: índice de retração cicatricial e aparência das feridas, e para a microscópica: histomorfometria de células mucosas, cromatóforos, neovasos, células inflamatórias, fibroblastos, fibras de colágeno e escamas. Os resultados mostraram que o índice de retração cicatricial foi significativamente maior no grupo suplementado, fato atribuído à contribuição do maior número de células inflamatórias, células mucosas, cromatóforos e a maior produção e organização das fibras de colágeno que foram significativamente maiores nos diferentes tempos, neste grupo. Os resultados indicam que o efeito nutricêutico da vitamina E promove o processo de reparação tecidual.
The vitamin E or tocoferol is an important nutrient that acts as an imunoestimulant in fishes. In the present study the effect of this vitamin was appraised in the process of induced wound healing in Nile tilapias Oreochromis niloticus supplemented with 0 and 450 mg levels of vitamin E. Examples from this specie were separated in two groups with 90 fishes in each, denominative control and supplemented, distributed in 18 boxes of water with capacity of 250L with 10 examples in each. The fishes were fed during 60 days with the experimental rations. After this period, all the fishes were anaesthetized and submitted the surgeries, where cutaneous wounds of standardized size and representative of the wound healing process were carried out by them surgically with removal of the epidermis and derme. The histomorphometric analysis was checked after three, seven, 14, 21 and 28 days post-wounding. They were used parameters for macroscopic as analysis rate of cicatricial retraction and appearance of the wounds, and for the microscopic one, histomorphometric of mucous cells, cromatophores, revascularization, inflammatory cells, presence of fibroblasts, fibers of collagen and scales. The results show the rate of retraction of the wound was significantly higher in the supplemented group. This higher rate of retraction results from the contribution of each microscopic parameter analyzed, such as inflammatory cells, mucous cells and cromatophores that were significantly higher in the different times, as the production and organization of collagen fibers. The results indicate that the vitamin E promotes wound healing process.
The vitamin E or tocoferol is an important nutrient that acts as an imunoestimulant in fishes. In the present study the effect of this vitamin was appraised in the process of induced wound healing in Nile tilapias Oreochromis niloticus supplemented with 0 and 450 mg levels of vitamin E. Examples from this specie were separated in two groups with 90 fishes in each, denominative control and supplemented, distributed in 18 boxes of water with capacity of 250L with 10 examples in each. The fishes were fed during 60 days with the experimental rations. After this period, all the fishes were anaesthetized and submitted the surgeries, where cutaneous wounds of standardized size and representative of the wound healing process were carried out by them surgically with removal of the epidermis and derme. The histomorphometric analysis was checked after three, seven, 14, 21 and 28 days post-wounding. They were used parameters for macroscopic as analysis rate of cicatricial retraction and appearance of the wounds, and for the microscopic one, histomorphometric of mucous cells, cromatophores, revascularization, inflammatory cells, presence of fibroblasts, fibers of collagen and scales. The results show the rate of retraction of the wound was significantly higher in the supplemented group. This higher rate of retraction results from the contribution of each microscopic parameter analyzed, such as inflammatory cells, mucous cells and cromatophores that were significantly higher in the different times, as the production and organization of collagen fibers. The results indicate that the vitamin E promotes wound healing process.
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Palavras-chave
Tilapia (Peixe), Vitamina E, Tocoferol, Oreochromis niloticus
Como citar
IWASHITA, Marina Keiko Pieroni. Influência da vitamina E na cinética do processo cicatricial induzido em tilápia do Nilo, Oreochromis niloticus. 2008. vii, 28 f. Dissertação (mestrado) - Universidade Estadual Paulista, Centro de Aqüicultura, 2008.