Investigação de marcadores protéicos do carcinoma oral
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Data
2004-11-03
Autores
Polachini, Giovana Mussi [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O carcinoma de células escamosas de cabeça e pescoço é um tumor agressivo e está relacionado com a exposição ao tabaco e ao álcool. A recorrência elevada, a variação na agressividade e as taxas baixas de sobrevivência dessa doença requerem nossos esforços para compreender a sua patogênese e para desenvolver melhores estratégias terapêuticas. No presente estudo, foi investigado se a análise proteômica pode identificar diferenças na expressão protéica entre carcinomas de cabeça e pescoço de diferentes graus de estadiamento. Para alcançar este objetivo, foram otimizados protocolos para extração de proteínas e a técnica de eletroforese bidimensional (2D) foi implantada no laboratório. Os padrões de perfil protéico obtidos foram analisados em oito amostras de carcinoma oral e duas de orofaringe (oito procedentes de tumores com metástases em linfonodos regionais e dois sem metástases) e em 17 amostras aparentemente normais de margens cirúrgicas. As variações qualitativas e quantitativas detectadas nos perfis protéicos de ambos os grupos foram consistentes e oito proteínas foram identificadas após análise por espectrometria de massa por dessorção/ionização a laser assistida por matriz (MALDI-TOF-TOF). Foram elas: albumina, alfa enolase, calgranulina B, galectina 7, mioglobina, miosina (cadeia leve 1), miosina (cadeia leve 2) e tropomiosina 2 (beta). Estas proteínas têm sido detectadas com expressão alterada por diferentes autores em carcinomas da cabeça e pescoço e também em outros tumores. Após validação, as proteínas identificadas podem revelar novos biomarcadores de prognóstico e alvos terapêuticos para tumores de cabeça e pescoço.
Head and neck squamous cell carcinoma is an aggressive cancer. It is closely related to the practice of tobacco smoking and alcohol abuse. The high recurrence, heterogeneous biological aggressiveness and low survival rates of this group of cancer require our efforts to understand the pathogenesis of the disease and to develop better therapeutic strategies. In the present study, we investigated whether proteome analysis can identify differences in protein expression between low and high stage head and neck carcinomas. To reach this aim we optimized protocols for protein extraction and established two-dimensional electrophoresis in the laboratory. Proteome profiling patterns were analyzed in eight oral and two oropharyngeal tumor samples (eight from carcinomas presenting lymph node metastasis and two from carcinomas with no regional metastasis) and in 17 apparently normal surgical margins. Qualitative and quantitative variations in both groups were consistent and eight proteins were identified by matrix assisted laser desorption/ionization - time of flight - time of flight (MALDI-TOFTOF) mass spectrometry. These proteins were: albumin, alpha enolase, calgranulin B, galectin 7, myoglobin, myosin (light chain 1), myosin (light chain 2) and tropomyosin beta chain. These proteins have shown to be with altered expression by different studies in head and neck cancers and also in other tumors. After validation, the proteins identified here could be novel biomarkers for prognosis and rational targets for head and neck tumors.
Head and neck squamous cell carcinoma is an aggressive cancer. It is closely related to the practice of tobacco smoking and alcohol abuse. The high recurrence, heterogeneous biological aggressiveness and low survival rates of this group of cancer require our efforts to understand the pathogenesis of the disease and to develop better therapeutic strategies. In the present study, we investigated whether proteome analysis can identify differences in protein expression between low and high stage head and neck carcinomas. To reach this aim we optimized protocols for protein extraction and established two-dimensional electrophoresis in the laboratory. Proteome profiling patterns were analyzed in eight oral and two oropharyngeal tumor samples (eight from carcinomas presenting lymph node metastasis and two from carcinomas with no regional metastasis) and in 17 apparently normal surgical margins. Qualitative and quantitative variations in both groups were consistent and eight proteins were identified by matrix assisted laser desorption/ionization - time of flight - time of flight (MALDI-TOFTOF) mass spectrometry. These proteins were: albumin, alpha enolase, calgranulin B, galectin 7, myoglobin, myosin (light chain 1), myosin (light chain 2) and tropomyosin beta chain. These proteins have shown to be with altered expression by different studies in head and neck cancers and also in other tumors. After validation, the proteins identified here could be novel biomarkers for prognosis and rational targets for head and neck tumors.
Descrição
Palavras-chave
Cancer - Aspectos geneticos, Cabeça - Aspectos genéticos, Pescoço - Aspectos genéticos, Carcinoma oral, Câncer de cabeça e pescoço, Oral carcinomas
Como citar
POLACHINI, Giovana Mussi. Investigação de marcadores protéicos do carcinoma oral. 2004. 136 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências, Letras e Ciências Exatas, 2004.