Spontaneous mammary carcinomas in female dogs: association between the immunohistochemical degree of aggressiveness of tumors, intensity of DNA damage and residues of pyrethroids

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dc.contributor.authorBrandão, Yara de Oliveira [UNESP]
dc.contributor.authorColodel, Marcia Moleta [UNESP]
dc.contributor.authorSilva, Glenda Nicioli da [UNESP]
dc.contributor.authorVexenat, Stephane Cássia [UNESP]
dc.contributor.authorFerreira, Isabelle
dc.contributor.authorSilva, Yamê Fabres Robaina Sancler da [UNESP]
dc.contributor.authorBulla, Camilo
dc.contributor.authorRocha, Noeme Sousa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade do Vale do Paraíba (UNIVAP)
dc.contributor.institutionMississippi State University
dc.date.accessioned2016-07-07T12:34:30Z
dc.date.available2016-07-07T12:34:30Z
dc.date.issued2013
dc.description.abstractDiagnosis and biological behavior of breast cancer of female dog represent one of the biggest challenges facing the Veterinarian in recent years. Due to its exponential growth and the degree of aggressiveness, the exact cause of this tumor is probably multifactorial and it is believed that may suffer influence from environmental factors. Among the suspected environmental contaminants are the pyrethroids. Aiming to investigate the participation of pyrethroids in tumorigenesis in female dogs, a study was conducted using 50 female dogs, 22 were positive for simple breast carcinoma (Group I), 18 with a diagnosis of complex breast carcinoma (Group II) and 10 negative (Group III) for breast cancer. In order to detect DNA damage, the Comet assay was performed on mammary samples of these animals, which also had samples submitted to the technique of High Performance Liquid Chromatography (HPLC), which aimed to quantify the concentration of pyrethroids. The results of HPLC of each animal were compared with those obtained by the Comet assay analysis of variance and the means were compared by the test groups “Student T” at the significance level of p  0.05. Despite presenting correlation between the amount of DNA damage and tumor aggressiveness, no statistical differences were found in the DNA damage of different histologic types of breast carcinoma. As for pyrethroids, even these were detected in 22% of tumor tissues and peritumoral fat, there was no difference in DNA damage between cells exposed and not exposed to environmental contaminant.en
dc.description.affiliationUniversidade do Vale do Paraíba, Laboratório de Espectroscopia Vibracional Biomédica, Instituto de Pesquisa e Desenvolvimento
dc.description.affiliationMississippi State University, Department of Pathobiology and Population Medicine, College of Veterinary Medicine
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Patologia, Faculdade de Medicina de Botucatu
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent133-137
dc.identifierhttp://dx.doi.org/10.4236/ojpathology.2013.33025
dc.identifier.citationOpen Journal of Pathology, v. 3, n. 3, p. 133-137, 2013.
dc.identifier.doi10.4236/ojpathology.2013.33025
dc.identifier.issn2164-6775
dc.identifier.lattes6077735918469284
dc.identifier.orcid0000-0002-8188-8149
dc.identifier.urihttp://hdl.handle.net/11449/140574
dc.language.isoeng
dc.relation.ispartofOpen Journal of Pathology
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectMammaryen
dc.subjectCarcinomasen
dc.subjectDNA damageen
dc.subjectPyrethroidsen
dc.titleSpontaneous mammary carcinomas in female dogs: association between the immunohistochemical degree of aggressiveness of tumors, intensity of DNA damage and residues of pyrethroidsen
dc.typeArtigo
unesp.author.lattes6077735918469284[8]
unesp.author.orcid0000-0002-8188-8149[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentClínica Veterináriapt
unesp.departmentPatologiapt
unesp.departmentReprodução Animal e Radiologia Veterináriapt

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Artigos - Clínica Veterinária - FMVZ
Artigos - Patologia - FMB
Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ