Production, purification, and characterization of a major penicillium glabrum xylanase using brewer's spent grain as substrate

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dc.contributor.authorKnob, Adriana
dc.contributor.authorBeitel, Susan Michelz
dc.contributor.authorFortkamp, Diana
dc.contributor.authorTerrasan, César Rafael Fanchini
dc.contributor.authorAlmeida, Alex Fernando de [UNESP]
dc.contributor.institutionMidwest State University
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:40Z
dc.date.available2014-05-27T11:29:40Z
dc.date.issued2013-06-12
dc.description.abstractIn recent decades, xylanases have been used in many processing industries. This study describes the xylanase production by Penicillium glabrum using brewer's spent grain as substrate. Additionally, this is the first work that reports the purification and characterization of a xylanase using this agroindustrial waste. Optimal production was obtained when P. glabrum was grown in liquid medium in pH 5.5, at 25 °C, under stationary condition for six days. The xylanase from P. glabrum was purified to homogeneity by a rapid and inexpensive procedure, using ammonium sulfate fractionation and molecular exclusion chromatography. SDS-PAGE analysis revealed one band with estimated molecular mass of 18.36 kDa. The optimum activity was observed at 60 °C, in pH 3.0. The enzyme was very stable at 50 °C, and high pH stability was verified from pH 2.5 to 5.0. The ion Mn2+ and the reducing agents β-mercaptoethanol and DTT enhanced xylanase activity, while the ions Hg2+, Zn2+, and Cu2+ as well as the detergent SDS were strong inhibitors of the enzyme. The use of brewer's spent grain as substrate for xylanase production cannot only add value and decrease the amount of this waste but also reduce the xylanase production cost. © 2013 Adriana Knob et al.en
dc.description.affiliationDepartment of Biological Sciences Midwest State University, Camargo Varela de Sá Street 03, 85040-080 Guarapuava, PR
dc.description.affiliationDepartment of Biology Midwest State University, Camargo Varela de Sá Street 03, 85040-080 Guarapuava, PR
dc.description.affiliationDepartment of Chemical Engineering Federal University of São Carlos Rodovia Washington Luís, km 235, SP-310, 13565-905 São Carlos, SP
dc.description.affiliationDepartment of Biochemistry and Microbiology São Paulo State University, 24-A Avenue 1515, 13506-900 Rio Claro, SP
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology São Paulo State University, 24-A Avenue 1515, 13506-900 Rio Claro, SP
dc.identifierhttp://dx.doi.org/10.1155/2013/728735
dc.identifier.citationBioMed Research International, v. 2013.
dc.identifier.doi10.1155/2013/728735
dc.identifier.file2-s2.0-84878682173.pdf
dc.identifier.issn2314-6133
dc.identifier.issn2314-6141
dc.identifier.scopus2-s2.0-84878682173
dc.identifier.urihttp://hdl.handle.net/11449/75641
dc.identifier.wosWOS:000319594700001
dc.language.isoeng
dc.relation.ispartofBioMed Research International
dc.relation.ispartofjcr2.583
dc.relation.ispartofsjr0,935
dc.relation.ispartofsjr0,935
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectammonium sulfate
dc.subjectcupric ion
dc.subjectmagnesium ion
dc.subjectmercaptoethanol
dc.subjectmercury
dc.subjectxylan endo 1,3 beta xylosidase
dc.subjectzinc ion
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectenzyme activity
dc.subjectenzyme analysis
dc.subjectenzyme purification
dc.subjectenzyme stability
dc.subjectenzyme substrate
dc.subjectenzyme synthesis
dc.subjectfungus culture
dc.subjectfungus growth
dc.subjectgrain
dc.subjection exclusion chromatography
dc.subjectmolecular size
dc.subjectnonhuman
dc.subjectPenicillium
dc.subjectPenicillium glabrum
dc.subjectpH
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectprocess optimization
dc.titleProduction, purification, and characterization of a major penicillium glabrum xylanase using brewer's spent grain as substrateen
dc.typeArtigo
dcterms.licensehttp://www.hindawi.com/journals/aaa/guidelines/
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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