Investigation of the interaction between Tc85-11 protein and antibody anti-T cruzi by AFM and amperometric measurements

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dc.contributor.authorFerreira, A. A. P.
dc.contributor.authorColli, W.
dc.contributor.authorAlves, M. J. M.
dc.contributor.authorOliveira, D. R.
dc.contributor.authorCosta, P. I.
dc.contributor.authorGuell, A. G.
dc.contributor.authorSanz, F.
dc.contributor.authorBenedetti, Assis Vicente [UNESP]
dc.contributor.authorYamanaka, Hideko [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniv Barcelona
dc.date.accessioned2014-05-20T13:24:23Z
dc.date.available2014-05-20T13:24:23Z
dc.date.issued2006-07-15
dc.description.abstractThis present work reports on development of an amperometric immunosensor for the diagnosis of Chagas' disease using a specific glycoprotein of the trypomastigote surface, which belongs to the Tc85-11 protein family of Trypanosoma cruzi (T cruzi). An atomically flat gold surface on a silicon substrate and gold screen-printed electrodes were functionalized with cystatrine and later activated with glutaraldehyde (GA), which was used to form covalent bonds with the purified recombinant antigen (Tc85-11). The antigen reacts with the antibody from the serum, and the affinity reaction was monitored directly using atomic force microscopy or amperometry through a secondary antibody tagged to peroxidase (HRP). Surface imaging allowed to us to differentiate the modification steps and antigen-antibody interaction allowed to distinguish the affinity reactions. In the amperometric immunosensor, peroxidase catalyses the L-2 formation in the presence of hydrogen peroxide and potassium iodide, and the reduction current intensity was measured at a given potential with screen-printed electrodes. The immunosensor was applied to sera of chagasic patients and patients having different systemic diseases. (c) 2006 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUNESP, Inst Chem, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Chem, BR-05508900 São Paulo, Brazil
dc.description.affiliationUNESP, Fac Pharmaceut Sci, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUniv Barcelona, CBEN, E-08028 Barcelona, Spain
dc.description.affiliationUnespUNESP, Inst Chem, BR-14801970 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Pharmaceut Sci, BR-14801970 Araraquara, SP, Brazil
dc.format.extent5046-5052
dc.identifierhttp://dx.doi.org/10.1016/j.electacta.2006.03.061
dc.identifier.citationElectrochimica Acta. Oxford: Pergamon-Elsevier B.V., v. 51, n. 24, p. 5046-5052, 2006.
dc.identifier.doi10.1016/j.electacta.2006.03.061
dc.identifier.issn0013-4686
dc.identifier.lattes1923726000036625
dc.identifier.lattes6720223715917381
dc.identifier.orcid0000-0002-3350-8308
dc.identifier.urihttp://hdl.handle.net/11449/7533
dc.identifier.wosWOS:000239253900005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofElectrochimica Acta
dc.relation.ispartofjcr5.116
dc.relation.ispartofsjr1,439
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectChagas' diseasept
dc.subjectTc85-11 proteinpt
dc.subjectamperometric immunosensorpt
dc.subjectAFMpt
dc.titleInvestigation of the interaction between Tc85-11 protein and antibody anti-T cruzi by AFM and amperometric measurementsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes1923726000036625
unesp.author.lattes6720223715917381[5]
unesp.author.lattes1769008264876945[8]
unesp.author.orcid0000-0002-0243-6639[8]
unesp.author.orcid0000-0002-3350-8308[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt

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