GLUCOCORTICOID-REGULATED GENE IN TRANSFORMED TO NORMAL PHENOTYPIC REVERSION

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dc.contributor.authorValentini, Sandro Roberto [UNESP]
dc.contributor.authorOliveira, MLS
dc.contributor.authorSasahara, R. M.
dc.contributor.authorArmelin, MCS
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:23:46Z
dc.date.available2014-05-20T15:23:46Z
dc.date.issued1994-02-01
dc.description.abstractGlucocorticoid hormones modulate the actions of peptide growth factors and constitute important therapeutic tools as anti-inflammatory and anti-tumor agents. The C6 rat glioma cell line responds to glucocorticoids with changes in morphology and growth block. The hyper-responsive ST1 cell variant displays a dramatic phenotypic reversion under the influence of these hormones. Thus, the transformed and tumorigenic cells reversibly change to a normal and non-tumorigenic phenotype. In addition, the cells also produce a C-type retrovirus. We used poly A(+) mRNA from ST1 cells that had been treated with hydrocortisone to generate a cDNA library that was then screened, by differential hybridization,for glucocorticoid-responsive cellular sequences. The retroviral genomic RNA was used to generate a viral-specific probe. Cross hybridization led to the isolation of at least 4 cDNA clones of which 3 are cellular sequences and one corresponds to a retroviral gene. These clones were characterized by DNA sequencing and Northern blot hybridization analysis.en
dc.description.affiliationUNIV SAO PAULO,INST QUIM,DEPT BIOQUIM,BR-01498970 SAO PAULO,SP,BRAZIL
dc.description.affiliationUNESP,FAC CIENCIAS FARMACEUT,DEPT CIENCIAS BIOL,BR-14801360 ARARAQUARA,SP,BRAZIL
dc.description.affiliationUnespUNESP,FAC CIENCIAS FARMACEUT,DEPT CIENCIAS BIOL,BR-14801360 ARARAQUARA,SP,BRAZIL
dc.format.extent541-546
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_issues&pid=0100-879X&lng=en&nrm=iso
dc.identifier.citationBrazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 27, n. 2, p. 541-546, 1994.
dc.identifier.issn0100-879X
dc.identifier.lattes5333250355049814
dc.identifier.urihttp://hdl.handle.net/11449/34474
dc.identifier.wosWOS:A1994MX60900067
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.relation.ispartofjcr1.492
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCDNA CLONINGpt
dc.subjectDIFFERENTIAL HYBRIDIZATIONpt
dc.subjectGLUCOCORTICOID-REGULATED GENESpt
dc.titleGLUCOCORTICOID-REGULATED GENE IN TRANSFORMED TO NORMAL PHENOTYPIC REVERSIONen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjmbr/iaboutj.htm
dcterms.rightsHolderAssociação Bras Divulg Cientifica
unesp.author.lattes5333250355049814
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentCiências Biológicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas