Karyotype changes in long-term cultured tick cell lines

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dc.contributor.authorKotsarenko, Kateryna
dc.contributor.authorVechtova, Pavlina
dc.contributor.authorLieskovska, Jaroslava
dc.contributor.authorFussy, Zoltan
dc.contributor.authorCabral-de-Mello, Diogo C. [UNESP]
dc.contributor.authorRego, Ryan O. M.
dc.contributor.authorAlberdi, Pilar
dc.contributor.authorCollins, Marisol
dc.contributor.authorBell-Sakyi, Lesley
dc.contributor.authorSterba, Jan
dc.contributor.authorGrubhoffer, Libor
dc.contributor.institutionCzech Acad Sci
dc.contributor.institutionUniv South Bohemia
dc.contributor.institutionMasaryk Univ
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Castilla La Mancha
dc.contributor.institutionUniv Liverpool
dc.date.accessioned2020-12-10T20:09:24Z
dc.date.available2020-12-10T20:09:24Z
dc.date.issued2020-08-10
dc.description.abstractTick cell lines are an easy-to-handle system for the study of viral and bacterial infections and other aspects of tick cellular processes. Tick cell cultures are often continuously cultivated, as freezing can affect their viability. However, the long-term cultivation of tick cells can influence their genome stability. In the present study, we investigated karyotype and genome size of tick cell lines. Though 16S rDNA sequencing showed the similarity between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n=28 chromosomes for parental Ixodes spp. ticks, and both increase and decrease in chromosome numbers were observed. For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ricinus cell lines IRE/CTVM19 and IRE/CTVM20 had modal chromosome numbers 48, 23 and 48, respectively. Also, the Ornithodoros moubata cell line OME/CTVM22 had the modal chromosome number 33 instead of 2n=20 chromosomes for Ornithodoros spp. ticks. All studied tick cell lines had a larger genome size in comparison to the genomes of the parental ticks. Thus, highly passaged tick cell lines can be used for research purposes, but possible differences in encoded genetic information and downstream cellular processes, between different cell populations, should be taken into account.en
dc.description.affiliationCzech Acad Sci, Inst Parasitol, Biol Ctr, Branisovska 31, Ceske Budejovice 37005, Czech Republic
dc.description.affiliationUniv South Bohemia, Fac Sci, Branisovska 1760, Ceske Budejovice 37005, Czech Republic
dc.description.affiliationMasaryk Univ, Cent European Inst Technol, Kamenice 5, Brno 62500, Czech Republic
dc.description.affiliationSao Paulo State Univ, Dept Gen & Appl Biol, Rio Claro, SP, Brazil
dc.description.affiliationUniv Castilla La Mancha, Reg Ctr Biomed Res CRIB, Ciudad Real Med Sch, Neuroplastic & Neurodegenerat Grp, Ciudad Real 13005, Spain
dc.description.affiliationUniv Liverpool, Inst Infect Vet & Ecol Sci, Dept Infect Biol & Microbiomes, Liverpool L3 5RF, Merseyside, England
dc.description.affiliationUnespSao Paulo State Univ, Dept Gen & Appl Biol, Rio Claro, SP, Brazil
dc.description.sponsorshipMinistry of Education, Youth and Sports of the Czech Republic INTER-ACTION project
dc.description.sponsorshipGrant Agency of the Czech Republic
dc.description.sponsorshipCzech research infrastructure for systems biology C4SYS
dc.description.sponsorshipUnited Kingdom Biotechnology and Biological Sciences Research Council
dc.description.sponsorshipMinistry of Education, Youth and Sports of the Czech Republic under the Projects CESNET
dc.description.sponsorshipCERIT-Scientific Cloud
dc.description.sponsorshipIdMinistry of Education, Youth and Sports of the Czech Republic INTER-ACTION project: LTARF 18021
dc.description.sponsorshipIdGrant Agency of the Czech Republic: 15-03044S
dc.description.sponsorshipIdGrant Agency of the Czech Republic: 18-27204S
dc.description.sponsorshipIdCzech research infrastructure for systems biology C4SYS: LM2015055
dc.description.sponsorshipIdUnited Kingdom Biotechnology and Biological Sciences Research Council: BB/P024270/1
dc.description.sponsorshipIdMinistry of Education, Youth and Sports of the Czech Republic under the Projects CESNET: LM2015042
dc.description.sponsorshipIdCERIT-Scientific Cloud: LM2015085
dc.format.extent12
dc.identifierhttp://dx.doi.org/10.1038/s41598-020-70330-5
dc.identifier.citationScientific Reports. London: Nature Publishing Group, v. 10, n. 1, 12 p., 2020.
dc.identifier.doi10.1038/s41598-020-70330-5
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11449/197202
dc.identifier.wosWOS:000561123700019
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.sourceWeb of Science
dc.titleKaryotype changes in long-term cultured tick cell linesen
dc.typeArtigo
dcterms.rightsHolderNature Publishing Group

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