Identification of sperm head subpopulations with defined pleiomorphic characteristics in ejaculates of captive Goeldi's monkeys (Callimico goeldii)

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dc.contributor.authorValle, R. R.
dc.contributor.authorArakaki, P. R.
dc.contributor.authorCarvalho, F. M. [UNESP]
dc.contributor.authorMuniz, J. A P C
dc.contributor.authorLeal, C. L V
dc.contributor.authorGarcía-Herreros, M.
dc.contributor.institutionUniversidade Paulista (UNIP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCentro Nacional de Primatas
dc.date.accessioned2014-05-27T11:28:17Z
dc.date.available2014-05-27T11:28:17Z
dc.date.issued2013-02-01
dc.description.abstractThe aim of this study was to evaluate the incidence of pleiomorphisms and its influence on the distribution of sperm morphometric subpopulations in ejaculates from the vulnerable Goeldi's monkey (Callimico goeldii) by using a combination of computerized analysis system and Principal Component Analysis (PCA) methods Each sperm head was measured for four primary spermatozoal head dimensional parameters (area [A (μm2)], perimeter [P (μm)], length [L (μm)] and width [W (μm)]) and three head shape derived parameters (ellipticity [(L/W)], elongation [(L-W)/(L+W)] and rugosity [(4πA/P2)]) Six separate subpopulations (SPs) were identified: SP1, constituted by very large, narrow and very elliptical spermatozoa (A=16.85±1.56μm2, W=2.75±0.42μm and ellipticity=2.16±0.24); SP2, characterized by average sized, short, wide and round spermatozoa (A=15.00±1.92μm2, L=5.06±0.49μm, W=3.51±0.31μm and ellipticity=1.44±0.15); SP3, represented by small, wide and slightly round spermatozoa (A=14.95±1.75μm2, W=3.47±0.29μm and ellipticity=1.48±0.14); SP4 included very small, short and very round spermatozoa (A=14.15±2.38μm2, L=4.90±0.57μm and elongation=0.18±0.05); SP5 consisted of average sized and slightly elliptical spermatozoa (A=15.14±1.72μm2 and ellipticity=1.49±0.14); and SP6 included large and round spermatozoa (A=16.30±1.62μm2 and elongation=0.19±0.04) There were differences in the sperm subpopulation distribution (P<0.001) among the five donors analyzed In conclusion, the results of the current study confirmed that the use of computer sperm analysis methods combined with PCA cluster analyses are useful methods to identify, classify, and characterize different sperm head morphometric subpopulations in neotropical primates Broadening our knowledge of C goeldii sperm morphometric abnormalities as well as developing reliable techniques for sperm evaluation may be essential for ex situ conservation of this threatened species © 2012 Elsevier B.V.en
dc.description.affiliationICS Universidade Paulista, São Paulo
dc.description.affiliationFMVZ Universidade de São Paulo, São Paulo
dc.description.affiliationUNESP Universidade Estadual Paulista, Jaboticabal
dc.description.affiliationCENP Centro Nacional de Primatas, Ananindeua
dc.description.affiliationFZEA Universidade de São Paulo, Pirassununga
dc.description.affiliationUnespUNESP Universidade Estadual Paulista, Jaboticabal
dc.format.extent93-102
dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2012.12.007
dc.identifier.citationAnimal Reproduction Science, v. 137, n. 1-2, p. 93-102, 2013.
dc.identifier.doi10.1016/j.anireprosci.2012.12.007
dc.identifier.issn0378-4320
dc.identifier.scopus2-s2.0-84880037144
dc.identifier.urihttp://hdl.handle.net/11449/74461
dc.identifier.wosWOS:000315762000014
dc.language.isoeng
dc.relation.ispartofAnimal Reproduction Science
dc.relation.ispartofjcr1.647
dc.relation.ispartofsjr0,704
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCallimico goeldii
dc.subjectGoeldi's monkey
dc.subjectPrincipal Component Analysis
dc.subjectSperm morphometry
dc.subjectSperm pleiomorphism
dc.subjectSperm subpopulations
dc.subjectThreatened
dc.subjectCallimico
dc.subjectHaplorhini
dc.subjectPrimates
dc.titleIdentification of sperm head subpopulations with defined pleiomorphic characteristics in ejaculates of captive Goeldi's monkeys (Callimico goeldii)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy

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