Osteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxilla

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dc.contributor.authorCerri, Paulo Sérgio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:12Z
dc.date.available2014-05-20T15:30:12Z
dc.date.issued2005-09-01
dc.description.abstractDuring bone formation, as in other tissues and organs, intense cellular proliferation and differentiation are usually observed. It has been described that programmed cell death, i.e., apoptosis, takes place in the control of the cellular population by removing of the excessive and damaged cells. Although it is generally accepted that apoptotic bodies are engulfed by professional phagocytes, the neighboring cells can also take part in the removal of apoptotic bodies. In the present study, regions of initial alveolar bone formation of rat molars were examined with the aim to verify whether osteoblasts are capable of engulfing apoptotic bodies, such as professional phagocytes. Rats aged 11-19 days were sacrificed and the maxillary fragments containing the first molar were removed and immersed in the fixative solution. The specimens fixed in glutaraldehyde-formaldehyde were processed for light microscopy and transmission electron microscopy. For the detection of apoptosis, the specimens were fixed in formaldehyde, embedded in paraffin, and submitted to the TUNEL method. The results revealed round/ovoid structures containing dense bodies on the bone surface in close contact to osteoblasts and in conspicuous osteoblast vacuoles. These round/ovoid structures showed also positivity to the TUNEL method, indicating that bone cells on the bone surface are undergoing apoptosis. Ultrathin sections showed images of apoptotic bodies being engulfed by osteoblasts. Occasionally, the osteoblasts exhibited large vacuoles containing blocks of condensed chromatin and remnants of organelles. Thus, these images suggest that osteoblasts are able to engulf and degrade apoptotic bodies. (c) 2005 Wiley-Liss, Inc.en
dc.description.affiliationSão Paulo State Univ, Sch Dent, Dept Morphol, Lab Histol & Embryol, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Sch Dent, Dept Morphol, Lab Histol & Embryol, BR-14801903 Araraquara, SP, Brazil
dc.format.extent833-840
dc.identifierhttp://dx.doi.org/10.1002/ar.a.20220
dc.identifier.citationAnatomical Record Part A-discoveries In Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 286A, n. 1, p. 833-840, 2005.
dc.identifier.doi10.1002/ar.a.20220
dc.identifier.fileWOS000231644400005.pdf
dc.identifier.issn1552-4884
dc.identifier.lattes3278495911207882
dc.identifier.lattes3278495911207882
dc.identifier.orcid0000-0001-5756-5828
dc.identifier.urihttp://hdl.handle.net/11449/39639
dc.identifier.wosWOS:000231644400005
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofAnatomical Record Part A-discoveries In Molecular Cellular and Evolutionary Biology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectosteoblastspt
dc.subjectapoptosispt
dc.subjectalveolar bonept
dc.subjectbone cellspt
dc.subjectbone formationpt
dc.titleOsteoblasts engulf apoptotic bodies during alveolar bone formation in the rat maxillaen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes3278495911207882
unesp.author.lattes3278495911207882
unesp.author.orcid0000-0001-5756-5828
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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