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Identification of two subfamilies of micropia transposable element in species of the repleta group of Drosophila

dc.contributor.authorde Almeida, L. M.
dc.contributor.authorCarareto, CMA
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:27:36Z
dc.date.available2014-05-20T15:27:36Z
dc.date.issued2004-06-01
dc.description.abstractThe occurrence, number of insertion sites and antisense RNA expression of micropia transposable element were studied in 26 species that belong to three subgroups (mercatorum, mulleri and hydei) of repleta group of Drosophila. Under high specific PCR, micropia sequences were detected in 11 species, but under less stringent condition, this retrotransposon was detected in all species. The widespread distribution of micropia suggests that this element was already present at the common ancestor of the repleta group of Drosophila. Southern blot analysis showed a variation from 0 to 17 different insertion sites and the occurrence of male-specific sequences. We found that the expression of the 1.0 kb micropia antisense RNA is variable among the species and tissues (soma and testis), which suggests that more than one mechanism regulates transposition in these species. Variation of amplification by PCR and of antisense RNA expression, as well as divergence of nucleotide sequences among the species allow us to suggest that at least two subfamilies of micropia transposable element are harbored by the genome of this species group.en
dc.description.affiliationUniv São Paulo, Dept Biol, Lab Mol Evolut Insects, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv São Paulo, Dept Biol, Lab Mol Evolut Insects, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent155-164
dc.identifierhttp://dx.doi.org/10.1023/B:GENE.0000040386.70086.71
dc.identifier.citationGenetica. Dordrecht: Kluwer Academic Publ, v. 121, n. 2, p. 155-164, 2004.
dc.identifier.doi10.1023/B:GENE.0000040386.70086.71
dc.identifier.issn0016-6707
dc.identifier.lattes3425772998319216
dc.identifier.orcid0000-0002-0298-1354
dc.identifier.urihttp://hdl.handle.net/11449/37559
dc.identifier.wosWOS:000223204200006
dc.language.isoeng
dc.publisherKluwer Academic Publ
dc.relation.ispartofGenetica
dc.relation.ispartofjcr1.366
dc.relation.ispartofsjr0,649
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectantisense RNApt
dc.subjectDrosophilapt
dc.subjectmicropiapt
dc.subjectmicropia subfamiliespt
dc.subjectnumber of insertion sitespt
dc.subjectrepleta grouppt
dc.subjectretrotransposonpt
dc.titleIdentification of two subfamilies of micropia transposable element in species of the repleta group of Drosophilaen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
dcterms.rightsHolderKluwer Academic Publ
unesp.author.lattes3425772998319216[2]
unesp.author.orcid0000-0002-0298-1354[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologia - IBILCEpt

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