Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil

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dc.contributor.authorPaz e Silva, Flavio Medeiros [UNESP]
dc.contributor.authorLopes, Raimundo Souza [UNESP]
dc.contributor.authorAraújo-Junior, João Pessoa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T07:12:10Z
dc.date.available2022-04-29T07:12:10Z
dc.date.issued2012-01-01
dc.description.abstractIn this study, we identified Cryptosporidium species and genotypes present in dairy cattle in the central region of São Paulo state, Brazil. Fecal specimens were collected from 200 animals (100 calves and 100 cows) in ten dairy farms. Fecal samples were examined using microscopic examination (ME), enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Cryptosporidium species and genotypes were determined by restriction fragment length polymorphism (RFLP) or DNA sequencing analysis of the SSU-rRNA and GP60 genes. The occurrence of Cryptosporidium spp. infection was 14% (28/200). The occurrence in calves (26%) was significantly higher than in cows (2%). Of the 27 Cryptosporidium-positive specimens submitted to genotyping, C. andersoni was identified in 23 (85.1%), C. bovis in three (11.1%), and the zoonotic C. parvum subtype IIaA15G2R1 in one (3.7%). The study demonstrates that Cryptosporidium spp. infection was common and widespread in dairy cattle in this region and that calves have a high prevalence of C. andersoni. Furthermore, the presence of C. parvum subtype IIaA15G2R1 indicates that dairy calves from this region should be considered a potential source of zoonotic Cryptosporidium oocysts.en
dc.description.affiliationLaboratório de Enfermidades Parasitárias Departamento de Clínica Veterinária Universidade Estadual Paulista - UNESP, Botucatu, SP
dc.description.affiliationLaboratório de Diagnóstico Molecular Departamento de Microbiologia-Imunologia Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, SP
dc.description.affiliationUnespLaboratório de Enfermidades Parasitárias Departamento de Clínica Veterinária Universidade Estadual Paulista - UNESP, Botucatu, SP
dc.description.affiliationUnespLaboratório de Diagnóstico Molecular Departamento de Microbiologia-Imunologia Instituto de Biociências, Universidade Estadual Paulista - UNESP, Botucatu, SP
dc.format.extent22-28
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria, v. 22, n. 1, p. 22-28, 2012.
dc.identifier.issn0103-846X
dc.identifier.scopus2-s2.0-84885476538
dc.identifier.urihttp://hdl.handle.net/11449/227219
dc.language.isoeng
dc.language.isopor
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria
dc.sourceScopus
dc.subjectCattle
dc.subjectCryptosporidium spp.
dc.subjectGenotyping
dc.subjectPCR-RFLP
dc.subjectSequencing
dc.titleIdentificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasilpt
dc.title.alternativeIdentification of Cryptosporidium species and genotypes in dairy cattle in Brazilen
dc.typeArtigo

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Artigos - Clínica Veterinária - FMVZ
Artigos - Microbiologia e Imunologia - IBB