Bacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals With Cleft Palate: Bacteriological Culture and Polymerase Chain Reaction

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dc.contributor.authorWeckwerth, Paulo Henrique
dc.contributor.authorMattias Franco, Adriana Terezinha de
dc.contributor.authorMagalhaes Lopes, Carlos Alberto de [UNESP]
dc.contributor.authorSantos, Fernando dos
dc.contributor.authorVillas Boas Weckwerth, Ana Carolina
dc.contributor.authorVivan, Rodrigo Ricci
dc.contributor.authorHungaro Duarte, Marco Antonio
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:08:52Z
dc.date.available2014-12-03T13:08:52Z
dc.date.issued2014-03-01
dc.description.abstractObjective: To characterize the microbial etiology of chronic suppurative otitis media comparing the methods of classical bacteriological culture and polymerase chain reaction.Design/Setting/Patients: Bacteriological analysis by classical culture and by molecular polymerase chain reaction of 35 effusion otitis samples from patients with cleft lip and palate attending the Hospital for Rehabilitation of Craniofacial Anomalies of the University of Sao Paulo, Bauru, Brazil.Interventions: Collection of clinical samples of otitis by effusion through the external auditory tube.Main Outcome Measure: Otolaryngologic diagnosis of chronic suppurative otitis media.Results: Positive cultures were obtained from 83% of patients. Among the 31 bacterial lineages the following were isolated. In order of decreasing frequency: Pseudomonas aeruginosa (54.9%), Staphylococcus aureus (25.9%), and Enterococcus faecalis (19.2%). No anaerobes were isolated by culture. The polymerase chain reaction was positive for one or more bacteria investigated in 97.1% of samples. Anaerobe lineages were detected by the polymerase chain reaction method, such as Fusobacterium nucleatum, Bacteroides fragilis, and Peptostreptococcus anaerobius.Conclusions: Patients with cleft lip and palate with chronic suppurative otitis media presented high frequency of bacterial infection in the middle ear. The classical bacteriological culture did not detect strict anaerobes, whose presence was identified by the polymerase chain reaction method.en
dc.description.affiliationUniv Sagrado Coracao, BR-17044160 Bauru, SP, Brazil
dc.description.affiliationSao Paulo State Univ Julio Mesquita Filho, Botucatu, SP, Brazil
dc.description.affiliationInst Lauro Souza Lima, Bauru, SP, Brazil
dc.description.affiliationBauru Dent Sch, Bauru, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ Julio Mesquita Filho, Botucatu, SP, Brazil
dc.format.extent145-153
dc.identifierhttp://dx.doi.org/10.1597/11-325
dc.identifier.citationCleft Palate-craniofacial Journal. Lawrence: Alliance Communications Group Division Allen Press, v. 51, n. 2, p. 145-153, 2014.
dc.identifier.doi10.1597/11-325
dc.identifier.issn1545-1569
dc.identifier.urihttp://hdl.handle.net/11449/111659
dc.identifier.wosWOS:000332664200003
dc.language.isoeng
dc.publisherAlliance Communications Group Division Allen Press
dc.relation.ispartofCleft Palate-craniofacial Journal
dc.relation.ispartofjcr1.262
dc.relation.ispartofsjr0,757
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbacteriological cultureen
dc.subjectchronic suppurative otitis mediaen
dc.subjectcleft lip and palateen
dc.subjectmicrobiologyen
dc.subjectpolymerase chain reactionen
dc.titleBacterial Pathogens Related to Chronic Suppurative Otitis Media in Individuals With Cleft Palate: Bacteriological Culture and Polymerase Chain Reactionen
dc.typeArtigo
dcterms.rightsHolderAlliance Communications Group Division Allen Press
unesp.author.orcid0000-0003-3051-737X[7]

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