Quantificação de subpopulações linfocitárias no sangue periférico de cães sadios adultos

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dc.contributor.authorSantana, Lucas A.S. [UNESP]
dc.contributor.authorMorais, Fabiana R.
dc.contributor.authorSantana, André M. [UNESP]
dc.contributor.authorVoorwald, Fabiana A. [UNESP]
dc.contributor.authorSantana, Aureo E. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:15:42Z
dc.date.available2018-12-11T17:15:42Z
dc.date.issued2017-07-01
dc.description.abstractFlow cytometry has established itself as a useful tool in veterinary practice, particularly for small animals practice. Such knowledge has made necessary the establishment of physiological values for different lymphocyte subpopulations, indispensable to understanding the dynamics of lymphoid cellularity in various pathological conditions. In this sense, the objective of this study was to determine, through cytometric technique, lymphocyte subsets of CD5+CD4+, CD5+CD8+ and CD21+ in four different breeds of domestic dogs, so to add information about the immunological profile of different breeds. A total of 40 adult dogs were used (2-7 years), being males and females of Beagles (G1, n=10), Golden Retrievers (G2, n=10), English Bulldogs (G3, n=10) and crossbreed dogs (G4, n=10). Blood samples were collected by jugular venipuncture, using vacuum flasks system (K2-EDTA). Hemogram and processing of samples for flow cytometry were performed within a maximum of 24 hours after blood collection. Samples were analyzed using FACSCanto device (Becton Dickinson, San Jose, CA, USA). FACSDiva software (Becton Dickinson, San Jose, CA, USA) was used to identify and quantify the CD5+ CD4+, CD5+CD8+, and CD21+, which provided the histogram and the respective table with the number of cells identified by immunophenotyping. The data obtained for T helper lymphocyte count, T cytotoxic/suppressor lymphocyte count and B lymphocytes were tabulated and submitted to analysis of variance by F test. Tukey test at 5% probability was used to compare means between the different breeds of dogs. The average values for CD5+CD8+ cell count in peripheral blood in G1, G2, G3 and G4 were of 155, 206, 544 and 503 cells/uL, respectively. The average values for CD5+CD4+ cell count in peripheral blood in G1, G2, G3 and G4 were of 746, 642, 855 and 1101 cells/uL, respectively. The average values for CD21 + cell count in peripheral blood for G1, G2, G3 and G4 were of 171, 299, 494 and 403 cells/uL, respectively. Therefore, The average number of cells obtained for the subsets of cytotoxic T lymphocytes was significantly higher (p < 0,05) in the British Bulldogs and crossbreed dogs compared to those found in Beagles and Golden Retrievers. Furthermore, it was observed that the average number of cells obtained for the subsets of B lymphocytes was significantly higher (p < 0,05) in English Bulldogs compared to that of Beagles.pt
dc.description.affiliationDepartamento de Medicina Veterinária Preventiva e Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n
dc.description.affiliationLaboratório de Citometria de Fluxo Faculdade de Ciências Farmacêuticas Universidade de São Paulo (USP), Av. Bandeirantes 3900, Vila Monte Alegre
dc.description.affiliationDepartamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n
dc.description.affiliationUnespDepartamento de Medicina Veterinária Preventiva e Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n
dc.description.affiliationUnespDepartamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane s/n
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent754-758
dc.identifierhttp://dx.doi.org/10.1590/s0100-736x2017000700017
dc.identifier.citationPesquisa Veterinaria Brasileira, v. 37, n. 7, p. 754-758, 2017.
dc.identifier.doi10.1590/s0100-736x2017000700017
dc.identifier.fileS0100-736X2017000700754.pdf
dc.identifier.issn1678-5150
dc.identifier.issn0100-736X
dc.identifier.scieloS0100-736X2017000700754
dc.identifier.scopus2-s2.0-85032510532
dc.identifier.urihttp://hdl.handle.net/11449/175410
dc.language.isoeng
dc.language.isopor
dc.relation.ispartofPesquisa Veterinaria Brasileira
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDogs
dc.subjectFlow citometry
dc.subjectLymphocytes
dc.titleQuantificação de subpopulações linfocitárias no sangue periférico de cães sadios adultospt
dc.title.alternativeLymphocyte subsets in peripheral blood of adult healthy dogsen
dc.typeArtigo
unesp.author.lattes8905659987198068[5]
unesp.author.orcid0000-0003-4539-1802[5]
unesp.departmentClínica e Cirurgia Veterinária - FCAVpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias
Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias