Ultrastructural analysis of bovine oocytes from ovarian follicles with different diameters

dc.contributor.authorLisboa, Lvia Aires
dc.contributor.authorAndrade, Evelyn Rabelo
dc.contributor.authorEsper, Cesar Roberto [UNESP]
dc.contributor.authorSeneda, Marcelo Marcondes
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:34:40Z
dc.date.available2014-05-20T15:34:40Z
dc.date.issued2011-01-01
dc.description.abstractIn vitro embryo production is an important technique for facilitating the reproduction of animals with high genetic merit. The greatest challenge for the reproducibility of this technique is the quality of the oocyte that is submitted for in vitro maturation. The aim of this work was to evaluate the ultrastructural characteristics of oocytes from follicles of different diameters using transmission electron microscopy. The animals were divided into 2 groups and were given a single i.m. injection of 250 IU FSH (Pluset, Serono, Italy). To synchronize the follicular growth, all follicles > 2 mm were aspirated during the estrous cycle, which was considered day zero (D0). Group 1 (G1; n = 4) received FSH on day 1 (D1), and the 2- to 5-mm follicles were aspirated on day 2 (D2). The animals in group 2 (G2; n = 5) received FSH on day 2 (D2), and their 10- to 15-mm follicles were aspirated on day 5 (D5). After aspiration, the oocytes from both groups were fixed and prepared for ultrastructural analysis. The oocytes analyzed from both groups had similar ultrastructural characteristics. The presence and distribution of organelles in the cytoplasm of the oocytes did not differ between groups, suggesting that, in relation to the ultrastructural characteristics, oocytes from 2 to 5 mm and 10 to 15 mm follicles are similar.en
dc.description.affiliationUniversidade Estadual de Londrina (UEL), UEL, Londrina, PR USA
dc.description.affiliationUniv Estadual Paulista, UNESP, Dept Med Vet Prevent & Reprod Anim, São Paulo, Brazil
dc.description.affiliationUEL, Ctr Ciencias Agr, Dept Clin Vet, Londrina, PR USA
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Dept Med Vet Prevent & Reprod Anim, São Paulo, Brazil
dc.format.extent1575-1582
dc.identifierhttp://dx.doi.org/10.5433/1679-0359.2011v32n4p1575
dc.identifier.citationSemina-ciencias Agrarias. Londrina: Universidade Estadual de Londrina (UEL), v. 32, n. 4, p. 1575-1582, 2011.
dc.identifier.doi10.5433/1679-0359.2011v32n4p1575
dc.identifier.fileWOS000297940500033.pdf
dc.identifier.issn1676-546X
dc.identifier.urihttp://hdl.handle.net/11449/42621
dc.identifier.wosWOS:000297940500033
dc.language.isoeng
dc.publisherUniversidade Estadual de Londrina (UEL)
dc.relation.ispartofSemina: Ciências Agrárias
dc.relation.ispartofjcr0.349
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectFollicle sizeen
dc.subjectOPUen
dc.subjectElectron microscopyen
dc.titleUltrastructural analysis of bovine oocytes from ovarian follicles with different diametersen
dc.typeArtigo
dcterms.licensehttp://www.uel.br/revistas/uel/index.php/semagrarias/about/editorialPolicies#openAccessPolicy
dcterms.rightsHolderUniversidade Estadual de Londrina (UEL)
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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