Inhibition of Breast Cancer Cell Migration by Cyclotides Isolated from Pombalia calceolaria

dc.contributor.authorPinto, Meri Emili F. [UNESP]
dc.contributor.authorNajas, Jhenny Z. G.
dc.contributor.authorMagalhães, Luma G.
dc.contributor.authorBobey, Antonio F. [UNESP]
dc.contributor.authorMendonça, Jacqueline N.
dc.contributor.authorLopes, Norberto P.
dc.contributor.authorLeme, Flávia M.
dc.contributor.authorTeixeira, Simone P.
dc.contributor.authorTrovó, Marcelo
dc.contributor.authorAndricopulo, Adriano D.
dc.contributor.authorKoehbach, Johannes
dc.contributor.authorGruber, Christian W.
dc.contributor.authorCilli, Eduardo Maffud [UNESP]
dc.contributor.authorBolzani, Vanderlan S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionMedical University of Vienna
dc.contributor.institutionUniversity of Queensland
dc.date.accessioned2018-12-11T17:37:10Z
dc.date.available2018-12-11T17:37:10Z
dc.date.issued2018-05-25
dc.description.abstractTwo new bracelet cyclotides from roots of Pombalia calceolaria with potential anticancer activity have been characterized in this work. The cyclotides Poca A and B (1 and 2) and the previously known CyO4 (3) were de novo sequenced by MALDI-TOF/TOF mass spectrometry (MS). The MS2 spectra were examined and the amino acid sequences were determined. The purified peptides were tested for their cytotoxicity and effects on cell migration of MDA-MB-231, a triple-negative breast cancer cell line. The isolated cyclotides reduced the number of cancer cells by more than 80% at 20 μM, and the concentration-related cytotoxic responses were observed with IC50 values of 1.8, 2.7, and 9.8 μM for Poca A (1), Poca B (2), and CyO4 (3), respectively. Additionally, the inhibition of cell migration (wound-healing assay) exhibited that CyO4 (3) presents an interesting activity profile, in being able to inhibit cell migration (50%) at a subtoxic concentration (2 μM). The distribution of these cyclotides in the roots was analyzed by MALDI imaging, demonstrating that all three compounds are present in the phloem and cortical parenchyma regions.en
dc.description.affiliationInstitute of Chemistry São Paulo State University-UNESP
dc.description.affiliationInstitute of Chemistry Federal University of Rio de Janeiro UFRJ
dc.description.affiliationComputational and Medicinal Chemistry Laboratory Physics Institute of São Carlos University of São Paulo-USP
dc.description.affiliationFaculty of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo-USP
dc.description.affiliationCenter for Physiology and Pharmacology Medical University of Vienna
dc.description.affiliationInstitute for Molecular Bioscience University of Queensland
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University-UNESP
dc.format.extent1203-1208
dc.identifierhttp://dx.doi.org/10.1021/acs.jnatprod.7b00969
dc.identifier.citationJournal of Natural Products, v. 81, n. 5, p. 1203-1208, 2018.
dc.identifier.doi10.1021/acs.jnatprod.7b00969
dc.identifier.issn1520-6025
dc.identifier.issn0163-3864
dc.identifier.scopus2-s2.0-85047539399
dc.identifier.urihttp://hdl.handle.net/11449/179892
dc.language.isoeng
dc.relation.ispartofJournal of Natural Products
dc.relation.ispartofsjr1,368
dc.relation.ispartofsjr1,368
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.titleInhibition of Breast Cancer Cell Migration by Cyclotides Isolated from Pombalia calceolariaen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.departmentBioquímica e Tecnologia - IQARpt
unesp.departmentQuímica Orgânica - IQARpt

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