Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

Costa Tonoli, Celisa Caldana; Vieira, Plinio Salmazo; Ward, Richard John; Arni, Raghuvir Krishnaswamy [UNESP]; Cavalcante de Oliveira, Arthur Henrique; Murakami, Mario Tyago

Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

Arquivos

WOS000271421800010.pdf (211.37 KB)

Data

2009-11-01

Autores

Costa Tonoli, Celisa Caldana

Vieira, Plinio Salmazo

Ward, Richard John

Arni, Raghuvir Krishnaswamy

Cavalcante de Oliveira, Arthur Henrique

Murakami, Mario Tyago

Editor

Wiley-Blackwell Publishing, Inc

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Nucleoside diphosphate kinases (NDKs; EC 2.7.4.6) play an essential role in the synthesis of nucleotides from intermediates in the salvage pathway in all parasitic trypanosomatids and their structural studies will be instrumental in shedding light on the biochemical machinery involved in the parasite life cycle and host-parasite interactions. In this work, NDKb from Leishmania major was overexpressed in Escherichia coli, purified to homogeneity and crystallized using the sitting-drop vapour-diffusion method. The NDK crystal diffracted to 2.2 angstrom resolution and belonged to the trigonal crystal system, with unit-cell parameters a = 114.2, c = 93.9 angstrom. Translation-function calculations yielded an unambiguous solution in the enantiomorphic space group P3(2)21.

Idioma

Inglês

Como citar

Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell Publishing, Inc, v. 65, p. 1116-1119, 2009.