Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

Carregando...
Imagem de Miniatura

Data

2009-11-01

Autores

Costa Tonoli, Celisa Caldana
Vieira, Plinio Salmazo
Ward, Richard John
Arni, Raghuvir Krishnaswamy [UNESP]
Cavalcante de Oliveira, Arthur Henrique
Murakami, Mario Tyago

Título da Revista

ISSN da Revista

Título de Volume

Editor

Wiley-Blackwell Publishing, Inc

Resumo

Nucleoside diphosphate kinases (NDKs; EC 2.7.4.6) play an essential role in the synthesis of nucleotides from intermediates in the salvage pathway in all parasitic trypanosomatids and their structural studies will be instrumental in shedding light on the biochemical machinery involved in the parasite life cycle and host-parasite interactions. In this work, NDKb from Leishmania major was overexpressed in Escherichia coli, purified to homogeneity and crystallized using the sitting-drop vapour-diffusion method. The NDK crystal diffracted to 2.2 angstrom resolution and belonged to the trigonal crystal system, with unit-cell parameters a = 114.2, c = 93.9 angstrom. Translation-function calculations yielded an unambiguous solution in the enantiomorphic space group P3(2)21.

Descrição

Palavras-chave

Como citar

Acta Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell Publishing, Inc, v. 65, p. 1116-1119, 2009.