DEVELOPMENT OF THE MAIN ORGANS IN BUFFALO EMBRYOS (Bubalus bubalis)

Página do item simplificado
dc.contributor.authorMorini, A. C.
dc.contributor.authorAmbrosio, C. E.
dc.contributor.authorMartins, D. S.
dc.contributor.authorMorini, J. C.
dc.contributor.authorFavaron, P. O.
dc.contributor.authorFranciolli, A. L. R.
dc.contributor.authorPereira, F. V. T.
dc.contributor.authorMinervino, A. H. H.
dc.contributor.authorMiglino, M. A.
dc.contributor.institutionFed Univ Western Para UFOPA
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T15:28:34Z
dc.date.available2018-11-26T15:28:34Z
dc.date.issued2015-12-01
dc.description.abstractTo give support for reproductive technology and to gain further information about buffalo morphology, we evaluated the morphological aspects of buffalo embryos and membranes of gestational age between 10-60 days. Ninety-six uteri at an early gestational stage from pregnant buffalos (Bubalus bubalis) were used. The pregnant horns were separated from the remainder of the uterus by means of clamps and were then perfused with 4% buffered paraformaldehyde or Bouin solution. Forty-eight hours after fixation by immersion, the pregnant uterine horn was incised, and the embryo with its fetal membranes was withdrawn. The embryos were distributed into groups according to their ages. Samples were also fixed in 10% formaldehyde and 2.5% glutaraldehyde and processed and stained, followed by examination by means of optical microscopy and immunohistochemistry. Despite some differences in the number of days, the development of the renal system in bovine and bubaline embryos is very similar, and these findings can be used to check the normality of embryo development by means of ultrasound and other imaging techniques. We identified the presence of pluripotent cells at different gestational ages and in different regions of the buffalo embryos and fetal membranes. During the gestation, the number of positive cells decreases significantly but these cells do not disappear completely. Hemangioblast cells in young buffalos embryos can be considered to be pluripotent cells: some of these cells will migrate to the embryo through the primitive intestine and will remain in genital organs and in another few colonies distributed in the embryo.en
dc.description.affiliationFed Univ Western Para UFOPA, Tv Vera Paz S-N, Santarem, PA, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, Sao Paulo, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Dept Histol & Embryol, Dracena Campus, Dracena, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Histol & Embryol, Dracena Campus, Dracena, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent429-439
dc.identifier.citationBuffalo Bulletin. Bangkok: Int Buffalo Information Ctr, v. 34, n. 4, p. 429-439, 2015.
dc.identifier.issn0125-6726
dc.identifier.urihttp://hdl.handle.net/11449/158673
dc.identifier.wosWOS:000368473500008
dc.language.isoeng
dc.publisherInt Buffalo Information Ctr
dc.relation.ispartofBuffalo Bulletin
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmorphology
dc.subjectriver buffalo
dc.subjectanatomy
dc.subjectelectron microscopy
dc.subjectimmunohistochemistry
dc.titleDEVELOPMENT OF THE MAIN ORGANS IN BUFFALO EMBRYOS (Bubalus bubalis)en
dc.typeArtigo
dcterms.rightsHolderInt Buffalo Information Ctr

Arquivos

Coleções

Artigos