Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
dc.contributor.author | Munchow, Eliseu A. | |
dc.contributor.author | Pankajakshan, Divya | |
dc.contributor.author | Albuquerque, Maria T. P. [UNESP] | |
dc.contributor.author | Kamocki, Krzysztof | |
dc.contributor.author | Piva, Evandro | |
dc.contributor.author | Gregory, Richard L. | |
dc.contributor.author | Bottino, Marco C. | |
dc.contributor.institution | Indiana Univ | |
dc.contributor.institution | Universidade Federal de Pernambuco (UFPE) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2018-11-26T15:31:38Z | |
dc.date.available | 2018-11-26T15:31:38Z | |
dc.date.issued | 2016-11-01 | |
dc.description.abstract | This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0A degrees). Increasing CaO concentrations progressively reduced the mechanical properties (p aecurrency sign 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. CaO-loaded electrospun matrices show potential for applications in bone tissue engineering. | en |
dc.description.affiliation | Indiana Univ, Sch Dent, Dept Biomed & Appl Sci, Div Dent Biomat, 1121 W Michigan St, Indianapolis, IN 46202 USA | |
dc.description.affiliation | Fed Univ Pelotas UFPel, Sch Dent, BR-96065560 Pelotas, RS, Brazil | |
dc.description.affiliation | Univ Estadual Paulista, Grad Program Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista, Grad Program Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, Brazil | |
dc.description.sponsorship | International Development Funds (IDF) Grant from Indiana University Purdue University (IUPUI/OVCR) | |
dc.description.sponsorship | IU School of Dentistry | |
dc.description.sponsorship | NIH-NIDCR | |
dc.description.sponsorship | Indiana Clinical and Translational Sciences Institute | |
dc.description.sponsorshipId | NIH-NIDCR: DE023552 | |
dc.description.sponsorshipId | Indiana Clinical and Translational Sciences Institute: UL1 TR001108 | |
dc.format.extent | 1921-1933 | |
dc.identifier | http://dx.doi.org/10.1007/s00784-015-1671-5 | |
dc.identifier.citation | Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 8, p. 1921-1933, 2016. | |
dc.identifier.doi | 10.1007/s00784-015-1671-5 | |
dc.identifier.file | WOS000386501800008.pdf | |
dc.identifier.issn | 1432-6981 | |
dc.identifier.uri | http://hdl.handle.net/11449/159162 | |
dc.identifier.wos | WOS:000386501800008 | |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.relation.ispartof | Clinical Oral Investigations | |
dc.relation.ispartofsjr | 0,986 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | CaO | |
dc.subject | Nanofibers | |
dc.subject | Osteomyelitis | |
dc.subject | Electrospinning | |
dc.subject | Bacteria | |
dc.subject | MC3T3-E1 | |
dc.title | Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering | en |
dc.type | Artigo | |
dcterms.license | http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 | |
dcterms.rightsHolder | Springer | |
unesp.author.orcid | 0000-0002-5970-5158[5] | |
unesp.author.orcid | 0000-0001-8740-2464[7] |
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