Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering

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dc.contributor.authorMunchow, Eliseu A.
dc.contributor.authorPankajakshan, Divya
dc.contributor.authorAlbuquerque, Maria T. P. [UNESP]
dc.contributor.authorKamocki, Krzysztof
dc.contributor.authorPiva, Evandro
dc.contributor.authorGregory, Richard L.
dc.contributor.authorBottino, Marco C.
dc.contributor.institutionIndiana Univ
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T15:31:38Z
dc.date.available2018-11-26T15:31:38Z
dc.date.issued2016-11-01
dc.description.abstractThis study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0A degrees). Increasing CaO concentrations progressively reduced the mechanical properties (p aecurrency sign 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.en
dc.description.affiliationIndiana Univ, Sch Dent, Dept Biomed & Appl Sci, Div Dent Biomat, 1121 W Michigan St, Indianapolis, IN 46202 USA
dc.description.affiliationFed Univ Pelotas UFPel, Sch Dent, BR-96065560 Pelotas, RS, Brazil
dc.description.affiliationUniv Estadual Paulista, Grad Program Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Grad Program Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, Brazil
dc.description.sponsorshipInternational Development Funds (IDF) Grant from Indiana University Purdue University (IUPUI/OVCR)
dc.description.sponsorshipIU School of Dentistry
dc.description.sponsorshipNIH-NIDCR
dc.description.sponsorshipIndiana Clinical and Translational Sciences Institute
dc.description.sponsorshipIdNIH-NIDCR: DE023552
dc.description.sponsorshipIdIndiana Clinical and Translational Sciences Institute: UL1 TR001108
dc.format.extent1921-1933
dc.identifierhttp://dx.doi.org/10.1007/s00784-015-1671-5
dc.identifier.citationClinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 8, p. 1921-1933, 2016.
dc.identifier.doi10.1007/s00784-015-1671-5
dc.identifier.fileWOS000386501800008.pdf
dc.identifier.issn1432-6981
dc.identifier.urihttp://hdl.handle.net/11449/159162
dc.identifier.wosWOS:000386501800008
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofClinical Oral Investigations
dc.relation.ispartofsjr0,986
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCaO
dc.subjectNanofibers
dc.subjectOsteomyelitis
dc.subjectElectrospinning
dc.subjectBacteria
dc.subjectMC3T3-E1
dc.titleSynthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineeringen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.orcid0000-0002-5970-5158[5]
unesp.author.orcid0000-0001-8740-2464[7]

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