Biochemical, histopathological and clinical evaluation of delayed effects caused by methamidophos isoforms and TOCP in hens: Ameliorative effects using control of calcium homeostasis
dc.contributor.author | Emerick, Guilherme Luz [UNESP] | |
dc.contributor.author | Ehrich, Marion | |
dc.contributor.author | Jortner, Bernard S. | |
dc.contributor.author | Oliveira, Regina V. | |
dc.contributor.author | DeOliveira, Georgino H. [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade Federal de São Carlos (UFSCar) | |
dc.contributor.institution | Virginia Polytech Inst & State Univ | |
dc.date.accessioned | 2014-05-20T13:25:33Z | |
dc.date.available | 2014-05-20T13:25:33Z | |
dc.date.issued | 2012-12-08 | |
dc.description.abstract | This work evaluated the potential of the isoforms of methamidophos to cause organophosphorus-induced delayed neuropathy (OPIDN) in hens. In addition to inhibition of neuropathy target esterase (NTE) and acetylcholinesterase (AChE), calpain activation, spinal cord lesions and clinical signs were assessed. The isoforms (+)-, (+/-)- and (-)-methamidophos were administered at 50 mg/kg orally; tri-ortho-cresyl phosphate (TOCP) was administered (500 mg/kg, po) as positive control for delayed neuropathy. The TOCP hens showed greater than 80% and approximately 20% inhibition of NTE and AChE in hen brain, respectively. Among the isoforms of methamidophos, only the (+)-methamidophos was capable of inhibiting NTE activity (approximately 60%) with statistically significant difference compared to the control group. Calpain activity in brain increased by 40% in TOCP hens compared to the control group when measured 24h after dosing and remained high (18% over control) 21 days after dosing. Hens that received (+)-methamidophos had calpain activity 12% greater than controls. The histopathological findings and clinical signs corroborated the biochemical results that indicated the potential of the (+)-methamidophos to be the isoform responsible for OPIDN induction. Protection against OPIDN was examined using a treatment of 2 doses of nimodipine (1 mg/kg, i.m.) and one dose of calcium gluconate (5 mg/kg, iv.). The treatment decreased the effect of OPIDN-inducing TOCP and (+)-methamidophos on calpain activity, spinal cord lesions and clinical signs. (C) 2012 Elsevier B.V. All rights reserved. | en |
dc.description.affiliation | Univ Estadual Paulista UNESP, Sch Pharmaceut Sci, Dept Nat Act Principles & Toxicol, Araraquara, SP, Brazil | |
dc.description.affiliation | Universidade Federal de São Carlos (UFSCar), Dept Chem, São Carlos, SP, Brazil | |
dc.description.affiliation | Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA | |
dc.description.affiliationUnesp | Univ Estadual Paulista UNESP, Sch Pharmaceut Sci, Dept Nat Act Principles & Toxicol, Araraquara, SP, Brazil | |
dc.format.extent | 88-95 | |
dc.identifier | http://dx.doi.org/10.1016/j.tox.2012.08.002 | |
dc.identifier.citation | Toxicology. Clare: Elsevier B.V., v. 302, n. 1, p. 88-95, 2012. | |
dc.identifier.doi | 10.1016/j.tox.2012.08.002 | |
dc.identifier.issn | 0300-483X | |
dc.identifier.uri | http://hdl.handle.net/11449/8103 | |
dc.identifier.wos | WOS:000309642600012 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Toxicology | |
dc.relation.ispartofjcr | 3.265 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Chiral organophosphate pesticides | en |
dc.subject | Methamidophos | en |
dc.subject | Acetylcholinesterase | en |
dc.subject | Neuropathy target esterase | en |
dc.subject | Calpain | en |
dc.subject | Treatment of OPIDN | en |
dc.title | Biochemical, histopathological and clinical evaluation of delayed effects caused by methamidophos isoforms and TOCP in hens: Ameliorative effects using control of calcium homeostasis | en |
dc.type | Artigo | |
dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dcterms.rightsHolder | Elsevier B.V. | |
unesp.author.orcid | 0000-0002-5061-5957[4] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.department | Princípios Ativos Naturais e Toxicologia - FCF | pt |
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