Structure of human PNP complexed with ligands

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dc.contributor.authorCanduri, F.
dc.contributor.authorSilva, R. G.
dc.contributor.authordos Santos, D. M.
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorBasso, L. A.
dc.contributor.authorSantos, D. S.
dc.contributor.authorde Azevedo, W. F.
dc.contributor.institutionUniversidade Federal do Rio Grande do Sul (UFRGS)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionPontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
dc.date.accessioned2014-05-20T13:54:38Z
dc.date.available2014-05-20T13:54:38Z
dc.date.issued2005-07-01
dc.description.abstractPurine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. PNP is specific for purine nucleosides in the beta-configuration and exhibits a strong preference for purines containing a 6-keto group and ribosyl-containing nucleosides relative to the corresponding analogues. PNP was crystallized in complex with ligands and data collection was performed using synchrotron radiation. This work reports the structure of human PNP in complex with guanosine (at 2.80 angstrom resolution), 3' deoxyguanosine (at 2.86 angstrom resolution) and 8-azaguanine (at 2.85 angstrom resolution). These structures were compared with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved previously.en
dc.description.affiliationUFRGS, Dept Biol Mol & Biotecnol, Rede Brasileira Pesquisas TB, BR-91501970 Porto Alegre, RS, Brazil
dc.description.affiliationUNESP, Dept Fis, Programa Posgrad Biofis Mol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationInst Butantan, Ctr Appl Toxinol, BR-05503900 São Paulo, Brazil
dc.description.affiliationUNESP, Dept Biol, Inst Biosci, CEIS,Lab Struct Biol & Zoochem, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationPUCRS, Ctr Pesquisas Biol Mol & Func, BR-90619900 Porto Alegre, RS, Brazil
dc.description.affiliationUnespUNESP, Dept Fis, Programa Posgrad Biofis Mol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol, Inst Biosci, CEIS,Lab Struct Biol & Zoochem, BR-13506900 Rio Claro, SP, Brazil
dc.format.extent856-862
dc.identifierhttp://dx.doi.org/10.1107/S0907444905005421
dc.identifier.citationActa Crystallographica Section D-biological Crystallography. Oxford: Blackwell Publishing, v. 61, p. 856-862, 2005.
dc.identifier.doi10.1107/S0907444905005421
dc.identifier.issn0907-4449
dc.identifier.lattes9424175688206545
dc.identifier.lattes2901888624506535
dc.identifier.urihttp://hdl.handle.net/11449/19553
dc.identifier.wosWOS:000230113000003
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallography
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleStructure of human PNP complexed with ligandsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing
unesp.author.lattes9424175688206545
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-1308-8190[2]
unesp.author.orcid0000-0003-4971-463X[6]
unesp.author.orcid0000-0002-7363-8211[4]
unesp.author.orcid0000-0003-0903-2407[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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