PRODUCTION OF XYLANASE BY A NEW STRAIN OF Thermoascus aurantiacus: OBTAINMENT OF ENZYMATIC EXTRACT WITH REDUCED CELLULOLYTIC ACTIVITY FOR APPLICATION IN PULP AND PAPER INDUSTRIES

dc.contributor.authorCosta, Ana Carolina da
dc.contributor.authorScalabrini, Rodrigo Prudente
dc.contributor.authorSilvestre, Maria Alice
dc.contributor.authorRodrigues, Andre [UNESP]
dc.contributor.authorPaz, Marcelo Fossa da
dc.contributor.authorFonseca, Gustavo Graciano
dc.contributor.authorRibeiro Leite, Rodrigo Simoes
dc.contributor.institutionFed Univ Grande Dourados FCBA UFGD
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T16:48:23Z
dc.date.available2018-11-26T16:48:23Z
dc.date.issued2016-07-01
dc.description.abstractXylanases are useful in several industrial segments, including pulp and paper bleaching, animal feed, and bread-making processes. However, the industrial use of these enzymes is closely related to its production cost and its catalytic properties. The process of solid state fermentation enables the use of agro-industrial residues as substrates for microbial cultivation and enzymes production, reducing costs. In the present study, different cultivation parameters were evaluated for the xylanase production by the thermophilic fungus Thermoascus aurantiacus, by solid state fermentation, using agro-industrial residues as substrates. High production of xylanase (1701.9 U g(-1) of dry substrate) was obtained using wheat bran containing 65% of initial moisture, at 120 h of cultivation, and 45 degrees C. The xylanase showed optimal activity at pH 5.0 and 75 degrees C; its stability was maintained at pH 3.0-11.0. The enzyme retained its catalytic potential after 1 h, at 75 degrees C. The enzymatic extract produced under optimized conditions showed reduced activities of endoglucanase and FPase. Our results, including the xylanase production by T. aurantiacus in low-cost cultivation medium, high structural stability of the enzyme, and reduced cellulolytic activity, encourage the application of this enzymatic extract in pulp and paper bleaching processes.en
dc.description.affiliationFed Univ Grande Dourados FCBA UFGD, Fac Biol & Environm Sci, Lab Enzymol & Fermentat Proc, Dourados, MS, Brazil
dc.description.affiliationSao Paulo State Univ IB UNESP, Dept Biochem & Microbiol, Biosci Inst, Lab Fungal Ecol & Systemat, Rio Claro, SP, Brazil
dc.description.affiliationFed Univ Grande Dourados FCBA UFGD, Fac Biol & Environm Sci, Lab Bioengn, Dourados, MS, Brazil
dc.description.affiliationUnespSao Paulo State Univ IB UNESP, Dept Biochem & Microbiol, Biosci Inst, Lab Fungal Ecol & Systemat, Rio Claro, SP, Brazil
dc.description.sponsorshipFundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent1040-1048
dc.identifier.citationBioscience Journal. Uberlandia: Univ Federal Uberlandia, v. 32, n. 4, p. 1040-1048, 2016.
dc.identifier.issn1981-3163
dc.identifier.urihttp://hdl.handle.net/11449/161726
dc.identifier.wosWOS:000379770400023
dc.language.isoeng
dc.publisherUniv Federal Uberlandia
dc.relation.ispartofBioscience Journal
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectSolid State Fermentation
dc.subjectAgro-industrial residues
dc.subjectPhisico-chemical characterization
dc.titlePRODUCTION OF XYLANASE BY A NEW STRAIN OF Thermoascus aurantiacus: OBTAINMENT OF ENZYMATIC EXTRACT WITH REDUCED CELLULOLYTIC ACTIVITY FOR APPLICATION IN PULP AND PAPER INDUSTRIESen
dc.typeArtigo
dcterms.rightsHolderUniv Federal Uberlandia
unesp.author.lattes8538509657578022[4]
unesp.author.orcid0000-0002-4164-9362[4]

Arquivos