Meiotic arrest of sheep oocytes using roscovitine under different medium compositions

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dc.contributor.authorCrocomo, Leticia Ferrari [UNESP]
dc.contributor.authorVela Ulian, Carla Maria [UNESP]
dc.contributor.authorBranchini, Naiana da Silva [UNESP]
dc.contributor.authorSilva, Denise Theodoro da [UNESP]
dc.contributor.authorMarques Filho, Wolff Camargo [UNESP]
dc.contributor.authorLandim Alvarenga, Fernanda da Cruz [UNESP]
dc.contributor.authorBicudo, Sony Dimas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-10-21T13:09:59Z
dc.date.available2015-10-21T13:09:59Z
dc.date.issued2015-05-01
dc.description.abstractAttempts to maintain the meiotic arrest under suitable culture conditions have been performed in order to improve the quality of oocytes in vitro matured. So, our study aimed to evaluate the potential of roscovitine, an inhibitor of cyclin-dependent kinases, to reversibly arrest the meiosis of sheep oocytes in medium supplemented with gonadotropins and/or serum. Cumulus-oocyte complexes (COCs) were treated, for 20 h, with 75 mu M roscovitine (Rosco) in basic maturation medium (Basic) containing 10% fetal bovine serum (FBS) and 0.1 IU/mL FSH plus 0.1 IU/mL LH (Enriched). After this, COCs were in vitro matured (IVM) for a further 18 h in the presence of gonadotropins and serum. At 20 and 38 h of culture, cumulus expansion and nuclear maturation were assessed under stereomicroscope and by Hoechst 33342 staining, respectively. Our findings demonstrate that total cumulus expansion occurred only in medium containing gonadotropins and serum. In the presence of roscovitine, however, the cumulus expansion was inhibited in a not fully reversible manner, independently of medium composition. The GV rate in the Rosco FBS group (64.6%) was significantly higher than that of Rosco Enriched (51.8%); however, both did not differ from that observed in the Rosco Basic (56.2%). In all roscovitine treatments, the meiotic arrest was reversible after the additional culture for 18 h in inhibitor-free medium. Besides, significant proportion of oocytes from Basic, FBS and Enriched groups reached the MII stage after 20 and 38 h of culture. Therefore, we can infer that (i) gonadotropins and serum did not affect the reversible meiotic arrest promoted by roscovitine in sheep oocytes; (ii) the maximum efficiency of meiosis inhibition occurred in serum-supplemented medium; (iii) roscovitine action on cumulus cells was not fully reversible independently of medium composition.en
dc.description.affiliationUniversidade Estadual Paulista (UNESP) - Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Distrito de Rubião Junior s/n, CEP 18618-970 Botucatu, SP, Brazil
dc.description.affiliationUniversidade Estadual Paulista (UNESP) - Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, Distrito de Rubião Junior s/n, CEP 18618-970 Botucatu, SP, Brazil
dc.description.affiliationUniversidade Estadual Paulista (UNESP) - Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Distrito de Rubião Junior s/n, CEP 18618-970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) - Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Distrito de Rubião Junior s/n, CEP 18618-970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) - Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, Distrito de Rubião Junior s/n, CEP 18618-970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) - Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Distrito de Rubião Junior s/n, CEP 18618-970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/14041-5
dc.description.sponsorshipIdFAPESP: 2011/18843-9
dc.format.extent52-58
dc.identifierhttp://www.sciencedirect.com/science/article/pii/S0921448815001042
dc.identifier.citationSmall Ruminant Research. Amsterdam: Elsevier Science Bv, v. 126, p. 52-58, 2015.
dc.identifier.doi10.1016/j.smallrumres.2015.02.022
dc.identifier.issn0921-4488
dc.identifier.lattes8134606270913860
dc.identifier.urihttp://hdl.handle.net/11449/128454
dc.identifier.wosWOS:000355041500009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofSmall Ruminant Research
dc.relation.ispartofjcr0.974
dc.relation.ispartofsjr0,485
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectInhibitoren
dc.subjectNuclear maturationen
dc.subjectCumulus expansionen
dc.subjectGonadotropinen
dc.subjectSerumen
dc.subjectOvineen
dc.titleMeiotic arrest of sheep oocytes using roscovitine under different medium compositionsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes8134606270913860
unesp.author.orcid0000-0002-6174-3153[1]
unesp.author.orcid0000-0001-6868-0223[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ
Artigos - Saúde Pública - FMB
Artigos - Clínica Veterinária - FMVZ