Subcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseus

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dc.contributor.authorEspinha, L. M.
dc.contributor.authorGaspar, J. O.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:03:17Z
dc.date.available2014-05-20T14:03:17Z
dc.date.issued1999-01-01
dc.description.abstractElectron microscopy and immunolabelling with antiserum specific to cucumber mosaic virus coat protein were used to examine tobacco leaf cells infected by cucumber mosaic virus isolated from Catharanthus roseus (CMV-Cr). Crystalline and amorphous inclusions in the vacuoles were the most obvious cytological modifications seen. Immunogold labelling indicated that the crystalline inclusion was made up of virus particles and amorphous inclusions contained coat protein. Rows of CMV-Cr particles were found between membranes of dictyosomes, but membranous bodies and tonoplast-associated vesicles were not evident. Virus particles and/or free coat protein were easily detected in the cytoplasm by immunolabelling. No gold labelling was found within nuclei, chloroplasts and mitochondria.en
dc.description.affiliationUNESP, IBILCE, Dept Bot, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, IBILCE, Dept Bot, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent119-126
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/10643647
dc.identifier.citationCytobios. Cambridge: Faculty Press, v. 100, n. 394, p. 119-126, 1999.
dc.identifier.issn0011-4529
dc.identifier.urihttp://hdl.handle.net/11449/22296
dc.identifier.wosWOS:000085203300006
dc.language.isoeng
dc.publisherFaculty Press
dc.relation.ispartofCytobios
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcucumoviruspt
dc.subjectimmunolabellingpt
dc.subjectelectron microscopypt
dc.subjectVinca roseapt
dc.subjectcapsid proteinpt
dc.titleSubcellular localization of the coat protein in tobacco cells infected by cucumber mosaic virus isolated from Catharanthus roseusen
dc.typeArtigo
dcterms.rightsHolderFaculty Press
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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Artigos - Zoologia e Botânica - IBILCE