In vitro anthelmintic activity of psidium guajava hydroalcoholic extract against gastro-intestinal sheep nematodes

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dc.contributor.authorSilva, Luciana P.
dc.contributor.authorDebiage, Rafael R.
dc.contributor.authorBronzel-Júnior, João L. [UNESP]
dc.contributor.authorde Silva, Regildo M. G. [UNESP]
dc.contributor.authorMello-Peixoto, Erika C. T.
dc.contributor.institutionLaboratório de Controle Parasitológico Animal em Agroecológico e Sistemas Orgânicos de Produção
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:14:41Z
dc.date.available2021-06-25T10:14:41Z
dc.date.issued2020-01-01
dc.description.abstractTanniferous plants have been used for ruminants verminosis control and represent a possibility to minimize the pharmacological resistance against conventional antiparasitics. This study aimed to evaluate the antihelminthic activity of the hydroalcoholic extract of stem bark of guava tree (PgHA). It was performed the hatchability and larval migration inhibition assays to evaluate PgHA at the following concentrations 0.62, 1.25, 2.5 and 5.0 mg mL-1 and the control treatments. The total polyphenol, flavonoid and tannin contents were determined by phytochemical analysis, high performance liquid chromatography coupled to mass spectrometry. The antioxidant activity was evaluated by 1,1-diphenyl-2-picrylhydrazyl, ferric reducing antioxidant power and thiobarbituric acid reactive substances tests. It was also determinated total protein, intracellular H2 O2 and antioxidant activity of enzimes: glutathione S-transferase and superoxide dismutase. PgHA was able to inhibit both hatchability and larval migration, but only hatchability inhibition presented dose-dependent pattern. The antioxidant activity was demonstrated by linear regression with IC50 corresponding to 534.02 μg mL-1. The antiparasitic mechanism occurred through pro-oxidative activity by the increase of total proteins, intracellular H2 O2 and the lipid peroxidation products, as well as the increase of the enzymes above related. Thus, the PgHA showed antiparasitic activity in vitro.en
dc.description.affiliationUniversidade Estadual do Norte do Paraná (UENP) Departamento de Produção Animal Laboratório de Controle Parasitológico Animal em Agroecológico e Sistemas Orgânicos de Produção, s / n, Rodovia BR-369
dc.description.affiliationUniversidade Estadual Paulista (UNESP) Instituto de Química, Av. Prof. Francisco Degni, 55, Jardim Quitandinha
dc.description.affiliationUniversidade Estadual Paulista (UNESP) Faculdade de Ciências e Línguas de Assis Departamento de Biotecnologia Laboratório de Fitoterapia e Produtos Naturais, Av. Dom Antônio, 2100
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) Instituto de Química, Av. Prof. Francisco Degni, 55, Jardim Quitandinha
dc.description.affiliationUnespUniversidade Estadual Paulista (UNESP) Faculdade de Ciências e Línguas de Assis Departamento de Biotecnologia Laboratório de Fitoterapia e Produtos Naturais, Av. Dom Antônio, 2100
dc.format.extent1-15
dc.identifierhttp://dx.doi.org/10.1590/0001-3765202020190074
dc.identifier.citationAnais da Academia Brasileira de Ciencias, v. 92, p. 1-15.
dc.identifier.doi10.1590/0001-3765202020190074
dc.identifier.fileS0001-37652020000400734.pdf
dc.identifier.issn1678-2690
dc.identifier.issn0001-3765
dc.identifier.scieloS0001-37652020000400734
dc.identifier.scopus2-s2.0-85094221856
dc.identifier.urihttp://hdl.handle.net/11449/205398
dc.language.isoeng
dc.relation.ispartofAnais da Academia Brasileira de Ciencias
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectGastrointestinal nematodes
dc.subjectGuava tree
dc.subjectMedicinal plants
dc.subjectParasite
dc.subjectTannins
dc.titleIn vitro anthelmintic activity of psidium guajava hydroalcoholic extract against gastro-intestinal sheep nematodesen
dc.typeArtigo
unesp.author.orcid0000-0002-0914-2094[1]
unesp.author.orcid0000-0001-8892-9348[2]
unesp.author.orcid0000-0001-5645-2485[3]
unesp.author.orcid0000-0002-0300-0247[4]
unesp.author.orcid0000-0002-9608-4282[5]

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