2-acetylphenothiazine protects L929 fibroblasts against UVB-induced oxidative damage

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dc.contributor.authorda Silva, Bruna Terra Alves
dc.contributor.authorPeloi, Karen Elaine
dc.contributor.authorXimenes, Valdecir Farias [UNESP]
dc.contributor.authorNakamura, Celso Vataru
dc.contributor.authorde Oliveira Silva Lautenschlager, Sueli
dc.contributor.institutionState University of Maringá
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:51:08Z
dc.date.available2021-06-25T10:51:08Z
dc.date.issued2021-03-01
dc.description.abstractUltraviolet B (UVB) light corresponds to 5% of ultraviolet radiation. It is more genotoxic and mutagenic than UVA and causes direct and indirect cellular damage through the generation of reactive oxygen species (ROS). Even after radiation, ROS generation may continue through activation of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) enzyme. Long-term exposure can progress to premature skin aging and photocarcinogenesis. To prevent damage that is caused by UVB radiation, several studies have focused on the topical administration of compounds that have antioxidant properties. 2-Acetylphenothiazine (ML171) is a potent and selective inhibitor of NOX1. The present study investigated the antioxidant potential and photoprotective ability of ML171 in UVB-irradiated L929 fibroblasts. ML171 had considerable antioxidant activity in both the DPPH• and xanthine/luminol/xanthine oxidase assays. ML171 did not induce cytotoxicity in L929 fibroblasts and increased the viability of UVB-irradiated cells. ML171 also inhibited ROS production, the enzymatic activity of NOX, depolarization of the mitochondrial membrane, and DNA damage. Additionally, ML171 protected cell membrane integrity and induced fibroblast migration. These results suggest that the incorporation of ML171 in topical administration systems may be a promising strategy to mitigate UVB-induced oxidative damage in L929 fibroblasts.en
dc.description.affiliationPost-Graduate Program in Pharmaceutical Sciences State University of Maringá, Maringá
dc.description.affiliationDepartment of Basic Health Sciences Maringa State University (UEM), Maringá
dc.description.affiliationDepartment of Chemistry Faculty of Sciences São Paulo State University (UNESP), Bauru
dc.description.affiliationUnespDepartment of Chemistry Faculty of Sciences São Paulo State University (UNESP), Bauru
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2014/50926-0
dc.description.sponsorshipIdFAPESP: 2019/18445-5
dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2021.112130
dc.identifier.citationJournal of Photochemistry and Photobiology B: Biology, v. 216.
dc.identifier.doi10.1016/j.jphotobiol.2021.112130
dc.identifier.issn1873-2682
dc.identifier.issn1011-1344
dc.identifier.scopus2-s2.0-85100386776
dc.identifier.urihttp://hdl.handle.net/11449/207232
dc.language.isoeng
dc.relation.ispartofJournal of Photochemistry and Photobiology B: Biology
dc.sourceScopus
dc.subject2-acetylphenothiazine
dc.subjectAntioxidant
dc.subjectOxidative stress
dc.subjectPhotoprotection
dc.title2-acetylphenothiazine protects L929 fibroblasts against UVB-induced oxidative damageen
dc.typeArtigo
unesp.departmentQuímica - FCpt

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