Chromosomal imbalances are uncommon in chagasic megaesophagus

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dc.contributor.authorBellini, Marilanda F. [UNESP]
dc.contributor.authorManzato, Antonio J. [UNESP]
dc.contributor.authorSilva, Ana E. [UNESP]
dc.contributor.authorVarella-Garcia, Marileila
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Colorado Denver
dc.date.accessioned2014-05-20T15:33:59Z
dc.date.available2014-05-20T15:33:59Z
dc.date.issued2010-02-17
dc.description.abstractBackground: Chagas' disease is a human tropical parasitic illness and a subset of the chronic patients develop megaesophagus or megacolon. The esophagus dilation is known as chagasic megaesophagus (CM) and one of the severe late consequences of CM is the increased risk for esophageal carcinoma (ESCC). Based on the association between CM and ESCC, we investigated whether genes frequently showing unbalanced copy numbers in ESCC were altered in CM by fluorescence in situ (FISH) technology.Methods: A total of 50 formalin-fixed, paraffin-embedded esophageal mucosa specimens (40 from Chagas megaesophagus-CM, and 10 normal esophageal mucosa-NM) were analyzed. DNA FISH probes were tested for FHIT, TP63, PIK3CA, EGFR, FGFR1, MYC, CDKN2A, YES1 and NCOA3 genes, and centromeric sequences from chromosomes 3, 7 and 9.Results: No differences between superficial and basal layers of the epithelial mucosa were found, except for loss of copy number of EGFR in the esophageal basal layer of CM group. Mean copy number of CDKN2A and CEP9 and frequency of nuclei with loss of PIK3CA were significantly different in the CM group compared with normal mucosa and marginal levels of deletions in TP63, FHIT, PIK3CA, EGFR, CDKN2A, YES and gains at PIK3CA, TP63, FGFR1, MYC, CDNK2A and NCOA3 were detected in few CM cases, mainly with dilation grades III and IV. All changes occurred at very low levels.Conclusions: Genomic imbalances common in esophageal carcinomas are not present in chagasic megaesophagus suggesting that these features will not be effective markers for risk assessment of ESCC in patients with chagasic megaesophagus.en
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Biol, São Paulo, Brazil
dc.description.affiliationUniv Colorado Denver, Dept Med Med Oncol, Aurora, CO USA
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Comp Sci & Stat, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Biol, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Comp Sci & Stat, São Paulo, Brazil
dc.description.sponsorshipNCI
dc.description.sponsorshipIdNCI: U01CA85070
dc.description.sponsorshipIdNCI: P30-CA46934
dc.description.sponsorshipIdNCI: P50 CA58187
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1186/1471-230X-10-20
dc.identifier.citationBmc Gastroenterology. London: Biomed Central Ltd., v. 10, p. 10, 2010.
dc.identifier.doi10.1186/1471-230X-10-20
dc.identifier.fileWOS000276342000001.pdf
dc.identifier.issn1471-230X
dc.identifier.urihttp://hdl.handle.net/11449/42380
dc.identifier.wosWOS:000276342000001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofBMC Gastroenterology
dc.relation.ispartofjcr2.731
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleChromosomal imbalances are uncommon in chagasic megaesophagusen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes2503906319038306[3]
unesp.author.orcid0000-0003-1491-8878[3]

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