Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation

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dc.contributor.authorRibeiro, Juliana S.
dc.contributor.authorMünchow, Eliseu A.
dc.contributor.authorBordini, Ester A. F. [UNESP]
dc.contributor.authorRodrigues, Nathalie S.
dc.contributor.authorDubey, Nileshkumar
dc.contributor.authorSasaki, Hajime
dc.contributor.authorFenno, John C.
dc.contributor.authorSchwendeman, Steven
dc.contributor.authorBottino, Marco C.
dc.contributor.institutionUniversity of Michigan
dc.contributor.institutionFederal University of Pelotas
dc.contributor.institutionFederal University of Rio Grande do Sul
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Michigan School of Dentistry
dc.date.accessioned2022-04-28T19:49:34Z
dc.date.available2022-04-28T19:49:34Z
dc.date.issued2022-01-01
dc.description.abstractThis study aimed at engineering cytocompatible and injectable antibiotic-laden fibrous microparticles gelatin methacryloyl (GelMA) hydrogels for endodontic infection ablation. Clindamycin (CLIN) or metronidazole (MET) was added to a polymer solution and electrospun into fibrous mats, which were processed via cryomilling to obtain CLIN-or MET-laden fibrous microparticles. Then, GelMA was modified with CLIN-or MET-laden microparticles or by using equal amounts of each set of fibrous microparticles. Morphological characterization of electrospun fibers and cryomilled particles was performed via scanning electron microscopy (SEM). The experimental hydrogels were further examined for swelling, degradation, and toxicity to dental stem cells, as well as antimicrobial action against endodontic pathogens (agar diffusion) and biofilm inhibition, evaluated both quantitatively (CFU/mL) and qualitatively via confocal laser scanning microscopy (CLSM) and SEM. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). The modification of GelMA with antibiotic-laden fibrous microparticles increased the hydrogel swelling ratio and degradation rate. Cell viability was slightly reduced, although without any significant toxicity (cell viability > 50%). All hydrogels containing antibiotic-laden fibrous microparticles displayed antibiofilm effects, with the dentin substrate showing nearly complete elimination of viable bacteria. Altogether, our findings suggest that the engineered injectable antibiotic-laden fibrous microparticles hydrogels hold clinical prospects for endodontic infection ablation.en
dc.description.affiliationDepartment of Cariology Restorative Sciences and Endodontics School of Dentistry University of Michigan
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry Federal University of Pelotas, Rio Grande do Sul
dc.description.affiliationDepartment of Conservative Dentistry School of Dentistry Federal University of Rio Grande do Sul, Rio Grande do Sul
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University, São Paulo
dc.description.affiliationDepartment of Biologic and Materials Sciences & Prosthodontics University of Michigan School of Dentistry
dc.description.affiliationDepartment of Pharmaceutical Sciences and the Biointerfaces Institute University of Michigan
dc.description.affiliationDepartment of Biomedical Engineering College of Engineering University of Michigan
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University, São Paulo
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNational Institute of Dental and Craniofacial Research
dc.description.sponsorshipNational Institutes of Health
dc.description.sponsorshipIdFAPESP: 2016/15674-5
dc.description.sponsorshipIdFAPESP: 2018/14257-7
dc.description.sponsorshipIdNational Institute of Dental and Craniofacial Research: K08DE023552
dc.description.sponsorshipIdNational Institutes of Health: K08DE023552
dc.description.sponsorshipIdNational Institute of Dental and Craniofacial Research: R01DE026578
dc.description.sponsorshipIdNational Institutes of Health: R01DE026578
dc.identifierhttp://dx.doi.org/10.3390/ijms23020971
dc.identifier.citationInternational Journal of Molecular Sciences, v. 23, n. 2, 2022.
dc.identifier.doi10.3390/ijms23020971
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85122821177
dc.identifier.urihttp://hdl.handle.net/11449/223253
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectAntibiotics
dc.subjectBiodegradation
dc.subjectCryomilling
dc.subjectDentistry
dc.subjectElectrospinning
dc.subjectEndodontics
dc.subjectFibrous particles
dc.subjectRegeneration
dc.titleEngineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablationen
dc.typeArtigo

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