Major carotenoid composition of Brazilian Valencia orange juice: Identification and quantification by HPLC

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dc.contributor.authorGama, JJT
dc.contributor.authorSylos, Célia Maria de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:36Z
dc.date.available2014-05-20T13:24:36Z
dc.date.issued2005-01-01
dc.description.abstractThe carotenoid composition of Brazilian Valencia orange juice was determined by open column chromatography (OCC) and high-performance liquid chromatography. Carotenoid pigments were extracted using acetone and saponified using 10% methanolic potassium hydroxide. Sixteen pigments were isolated by OCC and identified as alpha-carotene, zeta-carotene, beta-carotene, alpha-cryptoxanthin, beta-cryptoxanthin, lutein-5,6-epoxide, violaxanthin, lutein, antheraxanthin, zeaxanthin, luteoxanthin A, luteoxanthin B, mutatoxanthin A, mutatoxanthin B, auroxanthin B and trollichrome B. Thirteen carotenoid pigments were separated using a ternary gradient (acetonitrile-methanol-ethyl acetate) elution on a C-18 reversed-phase column. Among these, violaxanthin, lutein, zeaxanthin, beta-cryptoxanthin, zeta-carotene, alpha-carotene, and beta-carotene were quantified. The total carotenoid content was 12 +/- 6.7 mg/1, and the major carotenoids were lutein (23%), beta-cryptoxanthin (21%), and zeaxanthin (20%). 2005 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUNESP, FCF, Dept Food & Nutr, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, FCF, Dept Food & Nutr, BR-14801902 Araraquara, SP, Brazil
dc.format.extent899-903
dc.identifierhttp://dx.doi.org/10.1016/j.foodres.2005.03.008
dc.identifier.citationFood Research International. Amsterdam: Elsevier B.V., v. 38, n. 8-9, p. 899-903, 2005.
dc.identifier.doi10.1016/j.foodres.2005.03.008
dc.identifier.issn0963-9969
dc.identifier.lattes2531102466457738
dc.identifier.urihttp://hdl.handle.net/11449/7682
dc.identifier.wosWOS:000231402400013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFood Research International
dc.relation.ispartofjcr3.520
dc.relation.ispartofsjr1,472
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcarotenoid compositionpt
dc.subjectorange juicept
dc.subjectquantificationpt
dc.subjectHPLCpt
dc.subjectcitruspt
dc.subjectopen column chromatographypt
dc.titleMajor carotenoid composition of Brazilian Valencia orange juice: Identification and quantification by HPLCen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes2531102466457738
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Alimentos e Nutrição - FCFAR