Yield, changes in proteolysis, and sensory quality of Prato cheese produced with different coagulants
dc.contributor.author | Alves, L. S. | |
dc.contributor.author | Merheb-Dini, C. | |
dc.contributor.author | Gomes, E. [UNESP] | |
dc.contributor.author | Silva, R. da [UNESP] | |
dc.contributor.author | Gigante, M. L. | |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-12-03T13:11:05Z | |
dc.date.available | 2014-12-03T13:11:05Z | |
dc.date.issued | 2013-12-01 | |
dc.description.abstract | The objective of this research was to compare the effect of 2 fungal proteases, one that is already commercially established as a milk-clotting agent and another produced at the laboratory scale, on Prato cheese composition, protein and fat recovery, yield, and sensory characteristics. Cheeses were produced according to the traditional protocol, using protease from the fungus Thermomucor indicae-seudaticae N31 and commercial coagulant from Rhizomucor spp. as clotting agents. A 2 x 6 factorial design with 3 replications was performed: 2 levels of coagulants and 6 levels of storage time. After 5, 12, 19, 33, 43, and 53 d of refrigerated storage (12 degrees C), cheeses were monitored for proteolysis, firmness, and casein degradation by capillary electrophoresis. Sensory acceptance was evaluated after 29 d of manufacturing. The different coagulants did not statistically affect Prato cheese composition, protein and fat recovery, and yield. Both cheeses presented good sensory acceptance. Proteolysis increased and firmness decreased for both cheeses during the storage time, as expected for Prato cheese. Caseins were well separated by capillary electrophoresis and the results showed, with good resolution, that the cheeses exhibited similar protein hydrolysis profile. Both cheeses presented good sensory acceptance. The gathered data showed that the protease from T. indicae-seudaticae N31 presented similar action compared with the commercial enzyme, indicating its efficiency as clotting agent for Prato cheese manufacture. | en |
dc.description.affiliation | Univ Campinas UNICAMP, Fac Food Engn, BR-13083970 Campinas, SP, Brazil | |
dc.description.affiliation | Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias Letras & Ciencias Exatas IBILCE, Lab Biochem & Appl Microbiol, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias Letras & Ciencias Exatas IBILCE, Lab Biochem & Appl Microbiol, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorshipId | FAPESP: 11/51158-8 | |
dc.description.sponsorshipId | FAPESP: 11/50844-5 | |
dc.format.extent | 7490-7499 | |
dc.identifier | http://dx.doi.org/10.3168/jds.2013-7119 | |
dc.identifier.citation | Journal Of Dairy Science. New York: Elsevier Science Inc, v. 96, n. 12, p. 7490-7499, 2013. | |
dc.identifier.doi | 10.3168/jds.2013-7119 | |
dc.identifier.file | WOS000327112700010.pdf | |
dc.identifier.issn | 0022-0302 | |
dc.identifier.lattes | 7091241742851920 | |
dc.identifier.lattes | 9424175688206545 | |
dc.identifier.uri | http://hdl.handle.net/11449/112839 | |
dc.identifier.wos | WOS:000327112700010 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Journal of Dairy Science | |
dc.relation.ispartofjcr | 2.749 | |
dc.relation.ispartofsjr | 1,350 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | fungal enzyme | en |
dc.subject | yield | en |
dc.subject | sensory acceptance | en |
dc.subject | capillary electrophoresis | en |
dc.title | Yield, changes in proteolysis, and sensory quality of Prato cheese produced with different coagulants | en |
dc.type | Artigo | |
dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dcterms.rightsHolder | Elsevier B.V. | |
unesp.author.lattes | 7091241742851920 | |
unesp.author.lattes | 9424175688206545 | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto | pt |
unesp.department | Biologia - IBILCE | pt |
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