Isolamento de fungos produtores de enzimas pectinolíticas

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dc.contributor.authorGattas, Edwil Aparecida de Lucca [UNESP]
dc.contributor.authorCanguçu, U. M. [UNESP]
dc.contributor.authorRamos, W. S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:59Z
dc.date.available2014-05-27T11:20:59Z
dc.date.issued2003-12-01
dc.description.abstractSoil samples collected in the campus, UNESP, Araraquara, SP, were employed to isolate and characterize fungi strains with potential pectinolytic enzymes. These enzymes have arisen great interest due to its increasing application in the food industry. Two hundred forty six strains were isolated based on the appearance of colony on PDA medium, morphology (septate mycelia, nonseptate conidiophore, black conidia, and clublike spore-bearing head), after 48 h of growth at 30°C. Strains were selected in solid medium containing pectin citrus as sole carbon source and 0.5% rutenium red. The characterization of pectinolytic production was performed in solid culture and batch fermentation medium containing pectin citrus. The enzyme pectinolytic production was evaluated at 30°C, without agitation in 100 mL of medium containing 2% pectin citrus, 0.2% ammonium sulphate, 0.2% magnesium sulphate, and 0.05% potassium phosphate. The maximum pectinolytic activity (15U/mL) was observed in the medium after Aspergillus sp CFCF-0492 growth, while Aspergillus sp CFCF-CC1 showed the higher level of the final biomass. The pectinolytic activity is more preserved when the fungi-spores were maintained in agar-Czapeck medium.en
dc.description.affiliationDepartamento de Alimentos e Nutricao Faculdade de Ciencias Farmaceuticas UNESP, 14801-902 - Araraquara - SP
dc.description.affiliationIniciação Cientifica Faculdade de Ciencias Farmaceuticas UNESP, 14801-902 - Araraquara - SP
dc.description.affiliationUnespDepartamento de Alimentos e Nutricao Faculdade de Ciencias Farmaceuticas UNESP, 14801-902 - Araraquara - SP
dc.description.affiliationUnespIniciação Cientifica Faculdade de Ciencias Farmaceuticas UNESP, 14801-902 - Araraquara - SP
dc.format.extent33-37
dc.identifier.citationRevista de Ciencias Farmaceuticas, v. 24, n. 1, p. 33-37, 2003.
dc.identifier.issn0101-3793
dc.identifier.lattes4006598610021833
dc.identifier.scopus2-s2.0-4644282617
dc.identifier.urihttp://hdl.handle.net/11449/67564
dc.language.isopor
dc.relation.ispartofRevista de Ciencias Farmaceuticas
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectFermentation
dc.subjectFungus
dc.subjectPectinases
dc.subjectSoil
dc.subjectammonium sulfate
dc.subjectmagnesium sulfate
dc.subjectpectin
dc.subjectpolygalacturonase
dc.subjectpotassium dihydrogen phosphate
dc.subjectruthenium red
dc.subjectAspergillus
dc.subjectbiomass
dc.subjectBrazil
dc.subjectcarbon source
dc.subjectcitrus fruit
dc.subjectenzyme synthesis
dc.subjectfermentation
dc.subjectfungus culture
dc.subjectfungus isolation
dc.subjectnonhuman
dc.subjectsoil microflora
dc.titleIsolamento de fungos produtores de enzimas pectinolíticaspt
dc.title.alternativeIsolation and characterization of pectinolytic fungien
dc.typeArtigo
unesp.author.lattes4006598610021833[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Alimentos e Nutrição - FCFAR