Histochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817

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dc.contributor.authorNunes, Erika Takagi
dc.contributor.authorBraga, Adriane Araújo
dc.contributor.authorCamargo-Mathias, Maria Izabel
dc.contributor.institutionUniversidade Federal do Espírito Santo (UFES)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:30Z
dc.date.available2014-05-27T11:30:30Z
dc.date.issued2013-08-29
dc.description.abstractThis study provides histochemical data of the hepatopancreatic cells of adult female pink-shrimp (Farfantepenaeus brasiliensis) at two different developmental stages (those with developed gonads and those with exhausted gonads). The F. brasiliensis females were collected in seawater off the Guarapari coast, Espirito Santo, Brazil. Five cell types were identified in this digestive gland: B (vesicular), E (embryonic), F (fibrillar), M (basal) and R (resorptive). The digestive gland was stained with the following techniques: PAS/Alcian blue (for polysaccharides), bromophenol blue (for protein), von Kossa (for bound calcium) and Baker (for lipids). Acid glycoconjugates were found inside vacuoles in the R cells, while neutral polysaccharides were present in the B cells and near to the microvilli. In females with exhausted gonads polysaccharides were also seen in the intertubular spaces and inside the lumina of the tubules. The F and M cells were the most marked by the presence of large amounts of proteins observed in R cells and also inside the vacuoles of B cells. The bound calcium was mainly found in the F and M cells. The F cells showed strong positive staining for lipid while the R cell only stained weakly. The E cells did not react to any of the applied staining techniques. The similarities in the histochemical composition of these hepatopancreatic cells in females with developed gonads, compared to exhausted ones, is justified by the fact that transfer of these elements to the oocytes occurs, in significant quantity, only during the initial stages of gonadal development in F. brasiliensis. Also, they may be more related to the molt stage, as in the case of calcium salts. © 2013 Elsevier GmbH. All rights reserved.en
dc.identifierhttp://dx.doi.org/10.1016/j.acthis.2013.07.011
dc.identifier.citationActa Histochemica.
dc.identifier.doi10.1016/j.acthis.2013.07.011
dc.identifier.issn0065-1281
dc.identifier.issn1618-0372
dc.identifier.scopus2-s2.0-84882743647
dc.identifier.urihttp://hdl.handle.net/11449/76354
dc.identifier.wosWOS:000331671300033
dc.language.isoeng
dc.relation.ispartofActa Histochemica
dc.relation.ispartofjcr1.652
dc.relation.ispartofsjr0,661
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDigestive gland
dc.subjectFarfantepenaeus brasiliensis
dc.subjectHepatopancreas
dc.subjectHistochemistry
dc.titleHistochemical study of the hepatopancreas in adult females of the pink-shrimp Farfantepenaeus brasiliensis Latreille, 1817en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy

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