Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products

dc.contributor.authorSchneider, Marielle Cristina [UNESP]
dc.contributor.authorCarraro, Bianca Pierina
dc.contributor.authorCella, Doralice Maria [UNESP]
dc.contributor.authorMatiello, Rodrigo Rodrigues
dc.contributor.authorArtoni, Roberto Ferreira
dc.contributor.authorAlmeida, Mara Cristina
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Ponta Grossa (UEPG)
dc.date.accessioned2014-05-20T15:17:30Z
dc.date.available2014-05-20T15:17:30Z
dc.date.issued2007-01-01
dc.description.abstractIn this work we describe the cytogenetic analyses performed in specimens of Astylus variegatus (Germar, 1824) collected in two localities: one area of natural vegetation and one of agricultural crops, where agrochemical products were used. Astylus variegatus had karyotypes with 2n(male) = 16+Xy p and 2n (female) = 16+XXp, with exclusively metacentric chromosomes. Pachytene spermatocytes showed synapsed autosomal bivalents and non-associated sex chromosomes. In diplotene, the autosomal bivalents exhibited one or two terminal chiasmata and the Xy p had a typical parachute configuration. In meiotic cells of some specimens, an extra chromosome, interpreted as a B chromosome, was observed. C-banding showed constitutive heterochromatin in the pericentromeric region of all chromosomes, with the exception of the y p. Silver nitrate staining revealed one nucleolus organizer region (NOR) on the terminal region of the short arm of the second autosome pair. Silver staining of meiotic cells confirmed the NOR pattern detected in mitotic cells and revealed an argentophilous material on the Xy p. A cytogenetic comparison between the two populations of A. variegatus showed a statistically significant divergence (chi2 = 117.10; df = 1) in the number of aneuploid cells and a higher frequency of B chromosome in the population exposed to agrochemicals.en
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências Departamento de Biologia
dc.description.affiliationUniversidade Estadual de Ponta Grossa Departamento de Biologia Estrutural, Molecular e Genética
dc.description.affiliationUniversidade Estadual de Ponta Grossa Departamento de Fitotecnia e Fitossanidade
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências Departamento de Biologia
dc.format.extent640-645
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572007000400023
dc.identifier.citationGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 640-645, 2007.
dc.identifier.doi10.1590/S1415-47572007000400023
dc.identifier.fileS1415-47572007000400023.pdf
dc.identifier.issn1415-4757
dc.identifier.scieloS1415-47572007000400023
dc.identifier.urihttp://hdl.handle.net/11449/30496
dc.language.isoeng
dc.publisherSociedade Brasileira de Genética
dc.relation.ispartofGenetics and Molecular Biology
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,638
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectB chromosomeen
dc.subjectconstitutive heterochromatinen
dc.subjectkaryotypeen
dc.subjectmeiosisen
dc.subjectAg-NORsen
dc.titleAstylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical productsen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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