A preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHED

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dc.contributor.authorMarques, Nelson Pereira
dc.contributor.authorLopes, Camila Soares
dc.contributor.authorTeixeira Marques, Nadia Carolina
dc.contributor.authorCosme-Silva, Leopoldo [UNESP]
dc.contributor.authorOliveira, Thais Marchini
dc.contributor.authorDuque, Cristiane [UNESP]
dc.contributor.authorSakai, Vivien Thiemy
dc.contributor.authorCosta Hanemann, Joao Adolfo
dc.contributor.institutionFed Univ Alfenas UNIFAL MG
dc.contributor.institutionJose do Rosario Vellano Univ UNIFENAS
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-04T12:36:21Z
dc.date.available2019-10-04T12:36:21Z
dc.date.issued2019-04-01
dc.description.abstractThe aim of this preliminary study was to compare the effects of different energy densities from red and infrared low-level laser (LLL) on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED). SHED were irradiated with red laser (R) or infrared laser (IR) set with the following dosimetry: 1.2J/cm(2) (0.05J), 2.5J/cm(2) (0.1J), 5.0J/cm(2) (0.2J), and 7.5J/cm(2) (0.3J). Positive (C+) and negative (C-) control groups comprised non-irradiated cells. Data were analyzed by two-way ANOVA followed by Tukey's test (P<0.05). At 24- and 48-h period, group R5.0 showed significantly higher cell viability rates than R1.2 and R2.5. At 48h, R2.5 also revealed lower proliferation than R5.0. Comparing to the C+ group, R2.5 exhibited lower viability at 72h, and proliferation at 24 and 48h. Groups R1.2, IR1.2, and IR5.0 were less viable at 24h, while R1.2, IR2.5, and IR5.0 revealed lower proliferative capacity at 48h. Overall, our results showed that LLL can favor viability and proliferation of SHED, especially when cells receive red laser irradiation at 5.0J/cm(2). Therefore, according to this preliminary investigation, 5J/cm(2) applied by red LLL induced high rates of cell viability and proliferation, while the same irradiation dose using infrared laser led to negative effects. LLL irradiation with 1.2 and 2.5J/cm(2) was deleterious to metabolic activity and proliferation of SHED regardless of the type of laser. Further studies are necessary to gain in-depth knowledge about the effects of different wavelengths of LLL on SHED viability and proliferation.en
dc.description.affiliationFed Univ Alfenas UNIFAL MG, Sch Dent, Dept Clin & Surg, Rua Gabriel Monteiro da Silva 700, BR-37130000 Alfenas, MG, Brazil
dc.description.affiliationJose do Rosario Vellano Univ UNIFENAS, Sch Dent, Dept Pediat Dent, Alfenas, MG, Brazil
dc.description.affiliationSao Paulo State Univ, Aracatuba Sch Dent, Dept Restorat Dent Endodont, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Sao Paulo, Brazil
dc.description.affiliationSao Paulo State Univ, Aracatuba Sch Dent, Dept Pediat Dent, Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Aracatuba Sch Dent, Dept Restorat Dent Endodont, Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Aracatuba Sch Dent, Dept Pediat Dent, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPEMIG: APQ-04004-16
dc.description.sponsorshipIdCAPES: 88881.068437/2014-1
dc.format.extent465-471
dc.identifierhttp://dx.doi.org/10.1007/s10103-018-2615-5
dc.identifier.citationLasers In Medical Science. London: Springer London Ltd, v. 34, n. 3, p. 465-471, 2019.
dc.identifier.doi10.1007/s10103-018-2615-5
dc.identifier.issn0268-8921
dc.identifier.lattes5651874509493617
dc.identifier.orcid0000-0002-2575-279X
dc.identifier.urihttp://hdl.handle.net/11449/185538
dc.identifier.wosWOS:000461371500005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofLasers In Medical Science
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectStem cells
dc.subjectCell proliferation
dc.subjectCell survival
dc.subjectLaser therapy
dc.subjectLow-level light therapy
dc.titleA preliminary comparison between the effects of red and infrared laser irradiation on viability and proliferation of SHEDen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes5651874509493617[6]
unesp.author.orcid0000-0003-3460-3144[5]
unesp.author.orcid0000-0002-2575-279X[6]

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Artigos - Odontologia Restauradora - FOA
Artigos - Odontologia Infantil e Social - FOA